{"created":"2024-01-19T02:31:57.359969+00:00","id":2008885,"links":{},"metadata":{"_buckets":{"deposit":"1995714f-6f67-48b7-bfb9-9499570aae08"},"_deposit":{"created_by":17,"id":"2008885","owner":"17","owners":[17],"pid":{"revision_id":0,"type":"depid","value":"2008885"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:02008885","sets":["499:500:501"]},"author_link":[],"item_1615768549627":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_9_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2024-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"75","bibliographicPageStart":"61","bibliographicVolumeNumber":"262","bibliographic_titles":[{"bibliographic_title":"The Journal of Pathology","bibliographic_titleLang":"en"}]}]},"item_9_description_4":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Pancreatic stellate cells (PSCs) are stromal cells in the pancreas that play an important role in pancreatic pathology. In chronic pancreatitis (CP) and pancreatic ductal adenocarcinoma (PDAC), PSCs are known to get activated to form myofibroblasts or cancer-associated fibroblasts (CAFs) that promote stromal fibroinflammatory reactions. However, previous studies on PSCs were mainly based on the findings obtained using ex vivo expanded PSCs, with few studies that addressed the significance of in situ tissue-resident PSCs using animal models. Their contributions to fibrotic reactions in CP and PDAC are also lesser-known. These limitations in our understanding of PSC biology have been attributed to the lack of specific molecular markers of PSCs. Herein, we established Meflin (Islr), a glycosylphosphatidylinositol-anchored membrane protein, as a PSC-specific marker in both mouse and human by using human pancreatic tissue samples and Meflin reporter mice. Meflin-positive (Meflin+) cells contain lipid droplets and express the conventional PSC marker Desmin in normal mouse pancreas, with some cells also positive for Gli1, the marker of pancreatic tissue-resident fibroblasts. Three-dimensional analysis of the cleared pancreas of Meflin reporter mice showed that Meflin+ PSCs have long and thin cytoplasmic protrusions, and are localised on the abluminal side of vessels in the normal pancreas. Lineage tracing experiments revealed that Meflin+ PSCs constitute one of the origins of fibroblasts and CAFs in CP and PDAC, respectively. In these diseases, Meflin+ PSC-derived fibroblasts showed a distinctive morphology and distribution from Meflin+ PSCs in the normal pancreas. Furthermore, we showed that the genetic depletion of Meflin+ PSCs accelerated fibrosis and attenuated epithelial regeneration and stromal R-spondin 3 expression, thereby implying that Meflin+ PSCs and their lineage cells may support tissue recovery and Wnt/R-spondin signalling after pancreatic injury and PDAC development. Together, these data indicate that Meflin may be a marker specific to tissue-resident PSCs and useful for studying their biology in both health and disease.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_9_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"}]},"item_9_relation_43":{"attribute_name":"関連情報","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/path.6211","subitem_relation_type_select":"DOI"}}]},"item_9_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"\"This is the peer reviewed version of the following article: [Ando, R., Shiraki, Y., Miyai, Y., Shimizu, H., Furuhashi, K., Minatoguchi, S., Kato, K., Kato, A., Iida, T., Mizutani, Y., Ito, K., Asai, N., Mii, S., Esaki, N., Takahashi, M. and Enomoto, A. (2024), Meflin is a marker of pancreatic stellate cells involved in fibrosis and epithelial regeneration in the pancreas. J. Pathol., 262: 61-75. https://doi.org/10.1002/path.6211], which has been published in final form at [https://doi.org/10.1002/path.6211]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.\"","subitem_rights_language":"en"}]},"item_9_source_id_7":{"attribute_name":"収録物識別子","attribute_value_mlt":[{"subitem_source_identifier":"0022-3417","subitem_source_identifier_type":"PISSN"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ando, Ryota","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shiraki, Yukihiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Miyai, Yuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Shimizu, Hiroki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Furuhashi, Kazuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Minatoguchi, Shun","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kato, Katsuhiro","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kato, Akira","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Iida, Tadashi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mizutani, Yasuyuki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ito, Kisuke","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Asai, Naoya","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mii, Shinji","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Esaki, Nobutoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takahashi, Masahide","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Enomoto, Atsushi","creatorNameLang":"en"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","date":[{"dateType":"Available","dateValue":"2024-01-19"}],"displaytype":"detail","filename":"AndoR_2024_JPathol.pdf","filesize":[{"value":"1.4 MB"}],"format":"application/pdf","url":{"objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/2008885/files/AndoR_2024_JPathol.pdf"},"version_id":"e2d2bfbe-e30f-4b91-9b17-b8744174628c"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Meflin is a marker of pancreatic stellate cells involved in fibrosis and epithelial regeneration in the pancreas","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Meflin is a marker of pancreatic stellate cells involved in fibrosis and epithelial regeneration in the pancreas","subitem_title_language":"en"}]},"item_type_id":"40001","owner":"17","path":["501"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2024-01-19"},"publish_date":"2024-01-19","publish_status":"0","recid":"2008885","relation_version_is_last":true,"title":["Meflin is a marker of pancreatic stellate cells involved in fibrosis and epithelial regeneration in the pancreas"],"weko_creator_id":"17","weko_shared_id":-1},"updated":"2024-01-19T02:38:09.080312+00:00"}