| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2024-05-23 |
| タイトル |
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タイトル |
Membrane molecule bouncer regulates sperm binding activity in immature oocytes in the viviparous teleost species Poecilia reticulata (guppy) |
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言語 |
en |
| 著者 |
Yoshida, Junki
Tajika, Yuki
Uchida, Kazuko
Kuwahara, Makoto
Sano, Kaori
Suzuki, Takayuki
Hondo, Eiichi
Iida, Atsuo
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
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権利情報 |
"This is the peer reviewed version of the following article: [Yoshida, J., Tajika, Y., Uchida, K., Kuwahara, M., Sano, K., Suzuki, T., Hondo, E., & Iida, A. (2024). Membrane molecule bouncer regulates sperm binding activity in immature oocytes in the viviparous teleost species Poecilia reticulata (guppy). Development, Growth & Differentiation, 66(3), 194–204. https://doi.org/10.1111/dgd.12914], which has been published in final form at [https://doi.org/10.1111/dgd.12914]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited." |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
Generally, in vertebrates, the first step toward fertilization is the ovulation of mature oocytes, followed by their binding to sperm cells outside of the ovary. Exceptionally, the oocytes of poeciliid fish are fertilized by sperm cells within the follicle, and the developmental embryo is subsequently released into the ovarian lumen before delivery. In the present study, we aimed to identify the factor(s) responsible for intrafollicular fertilization in a viviparous teleost species, Poecilia reticulata (guppy). Sperm tracking analysis in this regard indicated that in this species, sperm cells reached immature oocytes including the germinal vesicle, and the insemination assay indicated that the immature oocytes robustly adhered to the sperm cells; similar binding was not observed in Danio rerio (zebrafish) and Oryzias latipes (medaka). We also identified the Ly6/uPAR protein bouncer as the factor responsible for the observed sperm binding activity of the immature oocytes in this species. The recombinant bouncer peptide acted as an inhibitory decoy for the sperm–oocyte binding in guppy. On the other hand, ectopic expression of guppy bouncer in zebrafish oocytes resulted in interspecific sperm–oocyte binding. These results argue that bouncer is responsible for sperm–immature oocyte binding. Our findings highlight the unique reproductive strategies of guppy fish and enhance our understanding of the diverse reproductive mechanisms in vertebrates. |
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言語 |
en |
| 出版者 |
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出版者 |
Wiley |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1111/dgd.12914 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0012-1592 |
| 書誌情報 |
en : Development, Growth & Differentiation
巻 66,
号 3,
p. 194-204,
発行日 2024-04
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| ファイル公開日 |
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日付 |
2025-04-01 |
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日付タイプ |
Available |
Yoshida_manuscript.pdf (7.4 MB)
