| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2024-05-24 |
| タイトル |
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タイトル |
Physcomitrium patens SUN2 Mediates MTOC Association with the Nuclear Envelope and Facilitates Chromosome Alignment during Spindle Assembly |
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言語 |
en |
| 著者 |
Yoshida, Mari W
Oguri, Noiri
Goshima, Gohta
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
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権利情報 |
This is a pre-copyedited, author-produced version of an article accepted for publication in [Plant and Cell Physiology] following peer review. The version of record [Mari W Yoshida, Noiri Oguri, Gohta Goshima, Physcomitrium patens SUN2 Mediates MTOC Association with the Nuclear Envelope and Facilitates Chromosome Alignment during Spindle Assembly, Plant and Cell Physiology, Volume 64, Issue 9, September 2023, Pages 1106–1117, https://doi.org/10.1093/pcp/pcad074] is available online at: https://doi.org/10.1093/pcp/pcad074. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
Plant cells lack centrosomes and instead utilize acentrosomal microtubule organizing centers (MTOCs) to rapidly increase the number of microtubules at the onset of spindle assembly. Although several proteins required for MTOC formation have been identified, how the MTOC is positioned at the right place is not known. Here, we show that the inner nuclear membrane protein SUN2 is required for MTOC association with the nuclear envelope (NE) during mitotic prophase in the moss Physcomitrium patens. In actively dividing protonemal cells, microtubules accumulate around the NE during prophase. In particular, regional MTOC is formed at the apical surface of the nucleus. However, microtubule accumulation around the NE was impaired and apical MTOCs were mislocalized in sun2 knockout cells. Upon NE breakdown, the mitotic spindle was assembled with mislocalized MTOCs. However, completion of chromosome alignment in the spindle was delayed; in severe cases, the chromosome was transiently detached from the spindle body. SUN2 tended to localize to the apical surface of the nucleus during prophase in a microtubule-dependent manner. Based on these results, we propose that SUN2 facilitates the attachment of microtubules to chromosomes during spindle assembly by localizing microtubules to the NE. MTOC mispositioning was also observed during the first division of the gametophore tissue. Thus, this study suggests that microtubule–nucleus linking, a well-known function of SUN in animals and yeast, is conserved in plants. |
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言語 |
en |
| 出版者 |
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出版者 |
Oxford University Press |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/pcp/pcad074 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0032-0781 |
| 書誌情報 |
en : Plant and Cell Physiology
巻 64,
号 9,
p. 1106-1117,
発行日 2023-09
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| ファイル公開日 |
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日付 |
2024-09-01 |
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日付タイプ |
Available |
SUN_ms230619clean.pdf (5.2 MB)
