| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2024-10-15 |
| タイトル |
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|
タイトル |
Novel air-liquid interface culture model to investigate stiffness-dependent behaviors of alveolar epithelial cells |
|
言語 |
en |
| 著者 |
Takahashi, Yuto
Ito, Satoru
Wang, Jungfeng
Kim, Jeonghyun
Matsumoto, Takeo
Maeda, Eijiro
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| アクセス権 |
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|
アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
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|
権利情報 |
© 2024. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
言語 |
en |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
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内容記述 |
Pulmonary alveoli are functional units in gas exchange in the lung, and their dysfunctions in lung diseases such as interstitial pneumonia are accompanied by fibrotic changes in structure, elevating the stiffness of extracellular matrix components. The present study aimed to test the hypothesis that such changes in alveoli stiffness induce functional alteration of epithelial cell functions, exacerbating lung diseases. For this, we have developed a novel method of culturing alveolar epithelial cells on polyacrylamide gel with different elastic modulus at an air-liquid interface. It was demonstrated that A549 cells on soft gels, mimicking the modulus of a healthy lung, upregulated mRNA expression and protein synthesis of surfactant protein C (SFTPC). By contrast, the cells on stiff gels, mimicking the modulus of the fibrotic lung, exhibited upregulation of SFTPC gene expression but not at the protein level. Cell morphology, as well as cell nucleus volume, were also different between the two types of gels. |
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言語 |
en |
| 出版者 |
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出版者 |
Elsevier |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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|
資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 出版タイプ |
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|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1016/j.bbrc.2024.149791 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0006291X |
| 書誌情報 |
en : Biochemical and Biophysical Research Communications
巻 708,
p. 149791,
発行日 2024-05-14
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| ファイル公開日 |
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|
日付 |
2025-05-14 |
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日付タイプ |
Available |
TakahashiMaeda2024_BBRC.pdf (3.7 MB)
