| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2024-12-10 |
| タイトル |
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タイトル |
Archaeal mevalonate pathway in the uncultured bacterium Candidatus Promineifilum breve belonging to the phylum Chloroflexota |
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言語 |
en |
| 著者 |
Kanno, Kosuke
Kuriki, Riko
Yasuno, Yoko
Shinada, Tetsuro
Ito, Tomokazu
Hemmi, Hisashi
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
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権利情報 |
'© [Kanno K, Kuriki R, Yasuno Y, Shinada T, Ito T, Hemmi H, 2024]. The definitive peer reviewed, edited version of this article is published in [Applied and Environmental Microbiology, 90, 8, 2024, 10.1128/aem.01106-24].' |
|
言語 |
en |
| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
The archaeal mevalonate pathway is a recently discovered modified version of the eukaryotic mevalonate pathway. This pathway is widely conserved in archaea, except for some archaeal lineages possessing the eukaryotic or other modified mevalonate pathways. Although the pathway seems almost exclusive to the domain Archaea, the whole set of homologous genes of the pathway is found in the metagenome-assembled genome sequence of an uncultivated bacterium, Candidatus Promineifilum breve, of the phylum Chloroflexota. To prove the existence of the archaea-specific pathway in the domain Bacteria, we confirmed the activities of the enzymes specific to the pathway, phosphomevalonate dehydratase and anhydromevalonate phosphate decarboxylase, because only these two enzymes are absent in closely related Chloroflexota bacteria that possess a different type of modified mevalonate pathway. The activity of anhydromevalonate phosphate decarboxylase was evaluated by carotenoid production via the archaeal mevalonate pathway reconstituted in Escherichia coli cells, whereas that of phosphomevalonate dehydratase was confirmed by an in vitro assay using the recombinant enzyme after purification and iron-sulfur cluster reconstruction. Phylogenetic analyses of some mevalonate pathway-related enzymes suggest an evolutionary route for the archaeal mevalonate pathway in Candidatus P. breve, which probably involves horizontal gene transfer events. |
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言語 |
en |
| 出版者 |
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出版者 |
American Society for Microbiology |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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|
関連識別子 |
https://doi.org/10.1128/aem.01106-24 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0099-2240 |
| 書誌情報 |
en : Applied and Environmental Microbiology
巻 90,
号 8,
p. e01106-24,
発行日 2024-08
|
manuscript5.pdf (5 MB)
