| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2024-12-12 |
| タイトル |
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|
タイトル |
Development of diazirine–alkyne tag for protein–ligand interaction analysis with photoaffinity labeling |
|
言語 |
en |
| 著者 |
Nakatani, Masatoshi
Ebihara, Shohei
Kita, Masaki
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| アクセス権 |
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アクセス権 |
open access |
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アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
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|
権利情報 |
This is a pre-copyedited, author-produced version of an article accepted for publication in [Chemistry Letters] following peer review. The version of record [Masatoshi Nakatani, Shohei Ebihara, Masaki Kita, Development of diazirine–alkyne tag for protein–ligand interaction analysis with photoaffinity labeling, Chemistry Letters, Volume 53, Issue 8, August 2024, upae142, https://doi.org/10.1093/chemle/upae142] is available online at: https://doi.org/10.1093/chemle/upae142. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
We have developed a diazirine–alkyne tag for target protein–ligand interaction analysis. A sulfonamide photoaffinity probe was readily prepared using copper-catalyzed azide–alkyne cycloaddition (CuAAC), which site-specifically formed a covalent bond with human carbonic anhydrase 1 (∼30% labeling yield). The photolabeled position (E58–H64 region) was determined based on the results of a tandem mass spectrometry (MS/MS) analysis of tryptic peptides and was supported by molecular modeling studies. Our method may be useful for determining unknown ligand-binding sites of various target biomolecules. |
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言語 |
en |
| 出版者 |
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出版者 |
Oxford University Press |
|
言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 出版タイプ |
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|
出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/chemle/upae142 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0366-7022 |
| 書誌情報 |
en : Chemistry Letters
巻 53,
号 8,
p. upae142,
発行日 2024-08-08
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| ファイル公開日 |
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|
日付 |
2025-08-08 |
|
日付タイプ |
Available |
CL_hCA1-SA_2024_r1_f.pdf (3.7 MB)
