| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2025-10-08 |
| タイトル |
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タイトル |
Equol-Producing Bacteria in the Chicken Intestine: PCR Analysis on the Bacteria Quantitatively Correlating With Equol Concentration in Daidzein-Fed Laying Hens |
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言語 |
en |
| 著者 |
Sakai, Sota
Furukawa, Kyohei
Namai, Fu
Nishiyama, Keita
Gyawali, Dipson
Goto, Tatsuhiko
Kikusato, Motoi
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| アクセス権 |
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アクセス権 |
embargoed access |
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アクセス権URI |
http://purl.org/coar/access_right/c_f1cf |
| 権利 |
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権利情報 |
"This is the peer reviewed version of the following article: [Sakai, S., Furukawa, K., Namai, F., Nishiyama, K., Gyawali, D., Goto, T. and Kikusato, M. (2025), Equol-Producing Bacteria in the Chicken Intestine: PCR Analysis on the Bacteria Quantitatively Correlating With Equol Concentration in Daidzein-Fed Laying Hens. Anim Sci J, 96: e70068. https://doi.org/10.1111/asj.70068], which has been published in final form at [https://doi.org/10.1111/asj.70068]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited." |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
Equol is a microbial metabolite from daidzein, an isoflavone, in the animal intestine. This study aimed to identify equol-producing bacteria in the intestines of chickens. Sixteen 181-day-old Hy-Line Brown laying hens were given a basal diet formulated excluding soy products for 2 weeks and subsequently given the diets supplemented with 0-, 100-, 1000-, and 10,000-ppm daidzein for 10 days. Plasma and egg yolk equol concentration was significantly increased with dietary daidzein supplementation. Gene abundance of two major bacterial enzymes catalyzing the conversion of daidzein to equol, dihydrodaidzein reductase, and tetrahydro daidzein reductase was measured using polymerase chain reaction (PCR) analysis, with the abundance increasing with dietary daidzein supplementation (p < 0.05). After sequencing the PCR amplicons, six possible bacteria were identified, and they were further identified and quantified using a specific primer for each bacterial gene. As a result, Adlercreutzia equolifaciens and Slackia equolifaciens were identified, and their bacterial relative abundance increased with dietary daidzein concentration and plasma equol levels (p < 0.05). A. equolifaciens and S. equolifaciens are known as equol-producing bacteria, and the present study was the first to show that these bacteria could also contribute to equol production in chickens. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Other |
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内容記述 |
First published: 31 May 2025 |
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言語 |
en |
| 出版者 |
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出版者 |
Wiley |
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言語 |
en |
| 言語 |
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言語 |
eng |
| 資源タイプ |
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資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
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タイプ |
journal article |
| 出版タイプ |
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出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1111/asj.70068 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
1344-3941 |
| 書誌情報 |
en : Animal science journal
巻 96,
号 1,
p. e70068,
発行日 2025
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| ファイル公開日 |
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日付 |
2026-05-31 |
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日付タイプ |
Available |
ASJ-2025-0103_R1_Proof_fl.pdf (1.2 MB)
