| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2025-11-26 |
| タイトル |
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|
タイトル |
Acupuncture alleviates the progression of knee osteoarthritis by inhibiting X-inactive specific transcript-mediated activation of the mechanosensitive ion channel Piezo1 signaling |
|
言語 |
en |
| 著者 |
Liu, Miaomiao
Zhang, Bimeng
Wang, Zhaoqin
Gou, Haixin
Zhao, Jimeng
Ye, Maoqing
Song, Changfeng
Lu, Xingang
Ji, Yan
Meng, Jie
Wu, Tao
Wu, Huangan
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| アクセス権 |
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|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
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|
権利情報Resource |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
|
言語 |
en |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
acupuncture |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
knee osteoarthritis |
| キーワード |
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|
主題Scheme |
Other |
|
主題 |
lncRNA X-inactive specific transcript |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
mechanosensitive ion channel |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Piezo1 protein |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
Acupuncture (AP) has been widely used in the treatment of knee osteoarthritis (KOA); however, its underlying molecular mechanisms remain elusive. This study aimed to identify whether acupuncture can relieve KOA progress via inhibiting X-inactive specific transcript (XIST)-mediated Piezo1 activation. The OA cells treated by interleukin (IL)-1β, and rat OA models induced by monosodium iodoacetate were established respectively. The expression of XIST, Piezo1, and extracellular matrix (ECM)-degeneration proteins were evaluated. Cell proliferation was detected by Cell Counting Kit-8 assay. The binding interaction between XIST and Piezo1 was performed using RNA binding protein immunoprecipitation assay (RIP). When XIST is knocked down, apoptosis is significantly reduced, and CHON-001 cell proliferation is increased in comparison to control and IL-1β-induced chondrocytes. Function tests revealed that both in vitro and in vivo, siRNA targeting XIST (si-XIST) increased cell proliferation while preventing apoptosis, ECM degradation, and the production of inflammatory factors. Additionally, we demonstrated that XIST and Piezo1 were bound together via insulin-like growth factor 2 messenger RNA (mRNA) binding proteins 2 (IGF2BP2), with Piezo1 serving as XIST’s target. The effects of XIST downregulation were reversed by Piezo1 activation via rescue tests conducted both in vitro and in vivo. Piezo1’s expression was reversed after XIST knockdown by IGF2BP2 overexpression. Our findings highlight the therapeutic potential of acupuncture in mitigating the progression of KOA by targeting the XIST-mediated activation of the Piezo1 pathway. By inhibiting this pathway, acupuncture may offer a promising approach to ameliorate the symptoms and slow the progression of KOA. |
|
言語 |
en |
| 出版者 |
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出版者 |
Nagoya University Graduate School of Medicine, School of Medicine |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| ID登録 |
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|
ID登録 |
10.18999/nagjms.87.4.644 |
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ID登録タイプ |
JaLC |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
URI |
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|
関連識別子 |
https://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/874.html |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0027-7622 |
| 収録物識別子 |
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収録物識別子タイプ |
EISSN |
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収録物識別子 |
2186-3326 |
| 書誌情報 |
en : Nagoya Journal of Medical Science
巻 87,
号 4,
p. 644-663,
発行日 2025-11
|
04_Liu.pdf (11.2 MB)
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