| Item type |
itemtype_ver1(1) |
| 公開日 |
2025-11-26 |
| タイトル |
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|
タイトル |
Rho-associated coiled-coil-containing protein kinase inhibitor Y-27632 promotes distraction osteogenesis healing by activating both osteoblasts and osteoclasts |
|
言語 |
en |
| 著者 |
Tsuboi, Makoto
Fujio, Masahito
Chang, Qi
Bian, Huiting
Wakasugi, Masashi
Liu, Yuqing
Hibi, Hideharu
|
| アクセス権 |
|
|
アクセス権 |
open access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_abf2 |
| 権利 |
|
|
言語 |
en |
|
権利情報Resource |
http://creativecommons.org/licenses/by-nc-nd/4.0/ |
|
権利情報 |
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
bone regeneration |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
distraction osteogenesis |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Rho-associated coiled-coil-containing protein kinase |
| キーワード |
|
|
主題Scheme |
Other |
|
主題 |
Y-27632 |
| 内容記述 |
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|
内容記述タイプ |
Abstract |
|
内容記述 |
Distraction osteogenesis can induce substantial skeletal tissue regeneration; however, the treatment duration is long, making the procedure suboptimal for clinical care. Rho-associated coiled-coil-containing protein kinase inhibitors might promote bone generation in distraction osteogenesis and shorten treatment durations. However, the relationship between Rho-associated coiled-coil-containing protein kinase inhibitors and distraction osteogenesis levels has not been described. In this study, we focused on osteoblasts and osteoclasts, which are essential for bone remodeling and regeneration. Proliferation assay, boyden chamber, and wound healing assay were performed on MC3T3-E1 and RAW264 cells. Osteogenic differentiation assay was performed on MC3T3-E1 cells, and osteoclast differentiation assay was performed on RAW264 cells. Samples collected from distraction osteogenesis model mice were subjected to micro-computed tomography analysis and tissue staining. We found that Y-27632, a Rho-associated coiled-coil-containing protein kinase inhibitor, promoted cell motility and affected cell differentiation and bone differentiation in MC3T3-E1 preosteoblast cells. We also found that Y-27632 promoted cell motility and osteoclast differentiation in the osteoclast precursor RAW264 cells. In vivo experiments showed that the local administration of Y-27632 in a mouse distraction osteogenesis model promoted bone formation and increased the number of osteoblasts and osteoclasts in the distraction osteogenesis gap. These findings demonstrate that Y-27632 promotes bone formation in a mouse distraction osteogenesis model. Collectively, the study findings suggest that Y-27632 can be used as a therapeutic agent to promote distraction osteogenesis healing. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Nagoya University Graduate School of Medicine, School of Medicine |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
departmental bulletin paper |
| 出版タイプ |
|
|
出版タイプ |
VoR |
|
出版タイプResource |
http://purl.org/coar/version/c_970fb48d4fbd8a85 |
| ID登録 |
|
|
ID登録 |
10.18999/nagjms.87.4.719 |
|
ID登録タイプ |
JaLC |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
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|
識別子タイプ |
URI |
|
|
関連識別子 |
https://www.med.nagoya-u.ac.jp/medlib/nagoya_j_med_sci/874.html |
| 助成情報 |
|
|
|
識別子タイプ |
Crossref Funder |
|
|
助成機関識別子 |
https://doi.org/10.13039/501100001691 |
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|
助成機関名 |
日本学術振興会 |
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|
言語 |
ja |
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|
助成機関名 |
Japan Society for the Promotion of Science |
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|
言語 |
en |
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|
研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K10090/ |
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|
研究課題番号 |
21K10090 |
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研究課題名 |
幹/前駆細胞のシーケンシャルな体内移動を基軸とした新しい骨再生治療の開発 |
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言語 |
ja |
| 助成情報 |
|
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|
識別子タイプ |
Crossref Funder |
|
|
助成機関識別子 |
https://doi.org/10.13039/501100001691 |
|
|
助成機関名 |
日本学術振興会 |
|
|
言語 |
ja |
|
|
助成機関名 |
Japan Society for the Promotion of Science |
|
|
言語 |
en |
|
|
研究課題番号URI |
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-19H03851/ |
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|
研究課題番号 |
19H03851 |
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研究課題名 |
可変培養環境下で合目的に調製した細胞外小胞によるカスケード的骨再生法の開発 |
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|
言語 |
ja |
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
0027-7622 |
| 収録物識別子 |
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収録物識別子タイプ |
EISSN |
|
収録物識別子 |
2186-3326 |
| 書誌情報 |
en : Nagoya Journal of Medical Science
巻 87,
号 4,
p. 719-729,
発行日 2025-11
|
09_Tsuboi.pdf (11.8 MB)
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