| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2026-01-29 |
| タイトル |
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タイトル |
Suicide substrate reaction-like modification of mouse serine racemase with L-serine |
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言語 |
en |
| 著者 |
Hata, Akari
Ito, Tomokazu
Mori, Hitoshi
Ogawa, Takuya
Kurihara, Tatsuo
Hemmi, Hisashi
Yoshimura, Tohru
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| アクセス権 |
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アクセス権 |
embargoed access |
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アクセス権URI |
http://purl.org/coar/access_right/c_f1cf |
| 権利 |
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|
権利情報 |
This is a pre-copyedited, author-produced version of an article accepted for publication in [Journal of biochemistry] following peer review. The version of record [Akari Hata, Tomokazu Ito, Hitoshi Mori, Takuya Ogawa, Tatsuo Kurihara, Hisashi Hemmi, Tohru Yoshimura, Suicide substrate reaction-like modification of mouse serine racemase with L-serine, The Journal of Biochemistry, Volume 177, Issue 6, June 2025, Pages 437–445, https://doi.org/10.1093/jb/mvaf019] is available online at: https://doi.org/10.1093/jb/mvaf019. |
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言語 |
en |
| 内容記述 |
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内容記述タイプ |
Abstract |
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内容記述 |
A pyridoxal 5’-phosphate-dependent fold-type II serine racemase (SR) is responsible for the synthesis of D-Ser, which serves as a co-agonist of N-methyl-D-aspartate glutamate receptor. In addition to racemization, SR catalyzes the dehydration of D- and L-Ser. SR is suggested to be involved in the D-Ser degradation in vivo, but this has not been confirmed. In this study, we found that mouse SR (mSR) underwent a suicide substrate reaction-like modification with its substrate, resulting in a remarkable change in its reaction specificity. mSR gradually lost its activity by the incubation with L- and D-Ser, but not completely. mSR was labelled with [14C]-L-Ser. ESI-MS analysis revealed that the molecular mass of SR increased by 84 Da by the incubation with L-Ser. Taken together with the results of previous crystallographic studies of fission yeast SR, we concluded that the active site lysine residue of mSR was modified with an α-aminoacrylate intermediate generated from L-Ser and converted to a lysinoalanine residue. The modification significantly decreased the racemization and L-Ser dehydration activities, while dramatically increased the D-Ser dehydration activity by the ~100 times reduction of the Km value. This is probably advantageous for the D-Ser degradation by mSR under physiological conditions. |
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言語 |
en |
| 出版者 |
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出版者 |
Oxford University Press |
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言語 |
en |
| 言語 |
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|
言語 |
eng |
| 資源タイプ |
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資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 出版タイプ |
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|
出版タイプ |
AM |
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出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
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関連タイプ |
isVersionOf |
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識別子タイプ |
DOI |
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関連識別子 |
https://doi.org/10.1093/jb/mvaf019 |
| 収録物識別子 |
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収録物識別子タイプ |
PISSN |
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収録物識別子 |
0021-924X |
| 書誌情報 |
en : Journal of biochemistry
巻 177,
号 6,
p. 437-445,
発行日 2025-06
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| ファイル公開日 |
|
|
日付 |
2026-06-01 |
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日付タイプ |
Available |
JB_177_6_437.pdf (1.4 MB)
