| アイテムタイプ |
itemtype_ver1(1) |
| 公開日 |
2026-02-03 |
| タイトル |
|
|
タイトル |
Impacts of Xyr1 transcription factor and culture conditions on regulation of plant polysaccharide-degrading enzyme genes in Fusarium graminearum |
|
言語 |
en |
| 著者 |
Qunash, Adeeb Haitham
Maeda, Kazuyuki
Zhu, Ziye
Kunitake, Emi
Ogawa, Masahiro
Nakajima, Yuichi
Kimura, Makoto
|
| アクセス権 |
|
|
アクセス権 |
embargoed access |
|
アクセス権URI |
http://purl.org/coar/access_right/c_f1cf |
| 権利 |
|
|
権利情報 |
This is a pre-copyedited, author-produced version of an article accepted for publication in [Journal of Applied Microbiology] following peer review. The version of record [Adeeb Haitham Qunash, Kazuyuki Maeda, Ziye Zhu, Emi Kunitake, Masahiro Ogawa, Yuichi Nakajima, Makoto Kimura, Impacts of Xyr1 transcription factor and culture conditions on regulation of plant polysaccharide-degrading enzyme genes in Fusarium graminearum, Journal of Applied Microbiology, Volume 137, Issue 1, January 2026, lxag006, https://doi.org/10.1093/jambio/lxag006] is available online at: https://doi.org/10.1093/jambio/lxag006. |
|
言語 |
en |
| 内容記述 |
|
|
内容記述タイプ |
Abstract |
|
内容記述 |
Aims: The xyr1 gene encodes a transcriptional regulator essential for the expression of several xylanase and other genes involved in arabinoxylan utilization. This study aims to examine the effects of xyr1 manipulations on carbon source utilization and plant polysaccharide-degrading enzyme activities in strains of the Fusarium graminearum species complex. Methods and Results: The xyr1 mutants were generated using strains MAFF 111233, MAFF 101551, and JCM 9873. The Δxyr1 mutant of MAFF 111233 showed better growth on beechwood xylan compared to other strains, indicating strain-specific variations in plant xylan utilization capabilities. Consistent with this result, the halo sizes on Remazol Brilliant Blue dyed xylan (RBB-xylan) plates of MAFF 111233 were minimally affected by xyr1 deletion. On wheat bran medium, the xyr1 overexpressor mutant of MAFF 111233 demonstrated a significant increase in endo-1,4-β-d-xylanase activity. Notably, α-l-arabinofuranosidase activity was either enhanced or diminished by xyr1 deletion, depending on the type of culture medium. Several α-l-arabinofuranosidase and endo-1,4-β-d-glucanase genes were identified as being more strongly transcribed in the Δxyr1 mutant than in the wild-type strain when cultured in liquid xylan medium. Conclusion: The genetic backgrounds and culture conditions of F. graminearum strains influenced how Xyr1 modulates the expression of secreted polysaccharide-degrading enzymes genes. |
|
言語 |
en |
| 出版者 |
|
|
出版者 |
Oxford University Press |
|
言語 |
en |
| 言語 |
|
|
言語 |
eng |
| 資源タイプ |
|
|
資源タイプresource |
http://purl.org/coar/resource_type/c_6501 |
|
タイプ |
journal article |
| 出版タイプ |
|
|
出版タイプ |
AM |
|
出版タイプResource |
http://purl.org/coar/version/c_ab4af688f83e57aa |
| 関連情報 |
|
|
関連タイプ |
isVersionOf |
|
|
識別子タイプ |
DOI |
|
|
関連識別子 |
https://doi.org/10.1093/jambio/lxag006 |
| 収録物識別子 |
|
|
収録物識別子タイプ |
PISSN |
|
収録物識別子 |
1364-5072 |
| 書誌情報 |
en : Journal of Applied Microbiology
巻 137,
号 1,
p. lxag006,
発行日 2026-01
|
| ファイル公開日 |
|
|
日付 |
2027-01-01 |
|
日付タイプ |
Available |
Qunash_et_al_2026.pdf (6.9 MB)
