@article{oai:nagoya.repo.nii.ac.jp:00022738,
 author = {YASUDA, KANAE},
 issue = {3},
 journal = {Nagoya Journal of Medical Science},
 month = {Jan},
 note = {In an attempt to evaluate gas chromatography for quantitative use, relative peak area ratio (RPAR) was studied under various analytical conditions and several problems in the quantitative analysis were elucidated. The loss was influenced by the sample size, structure of the steroid, retention time, operating temperature, loading effect and gas chromatographic appartaus. Problems relating to matters of instrumental design and column packing inclusive were of the most importance. In certain instances trimethylsilyl ethers were useful for avoiding these difficulties but these derivatives and even saturated hydrocarbons were not found to be recovered completely in gas chromatography. Of the various causes proposed for this phenomenon, adsorption appeared to be the most siginificant. When precise estimation is required, it should not be extended beyond a certain limit even by employing suitable operating conditions. Relatively reliable procedures for careful control of routine estimation are outlined. As a matter of course, the necessity for proper instrumentation should also be emphasized and the properties of any given equipment, analytical conditions and the accuracy of the obtained data should be checked with the RPAR curve.},
 pages = {191--230},
 title = {Gas Chromatographic Analysis of Steroid Hormones Part I. Problems in Quantitative Aspects of Gas Chromatography},
 volume = {29},
 year = {1967}
}