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アイテム
DNA conformational behavior and compaction in biomimetic systems: Toward better understanding of DNA packaging in cell
http://hdl.handle.net/2237/25148
http://hdl.handle.net/2237/25148a49e8ce2-34d2-4ca5-9d11-7aebcd26408d
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Zinchenko_AdvColloid_Interface_Sci.pdf ファイル公開：2018/06/01 (2.2 MB)
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| アイテムタイプ | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-12-05 | |||||
| タイトル | ||||||
| タイトル | DNA conformational behavior and compaction in biomimetic systems: Toward better understanding of DNA packaging in cell | |||||
| 言語 | en | |||||
| 著者 |
Zinchenko, Anatoly
× Zinchenko, Anatoly |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 権利情報 | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| 言語 | en | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DNA condensation and compaction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Crowding | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Confinement | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Artificial cell | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Single-molecule | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | In a living cell, long genomic DNA is strongly compacted and exists in the environment characterized by a dense macromolecular crowding, high concentrations of mono- and divalent cations, and confinement of ca. 10 μm size surrounded by a phospholipid membrane. Experimental modelling of such complex biological system is challenging but important to understand spatiotemporal dynamics and functions of the DNA in cell. The accumulated knowledge about DNA condensation/compaction in conditions resembling those in the real cell can be eventually used to design and construct partly functional “artificial cells” having potential applications in drug delivery systems, gene therapy, and production of synthetic cells. In this review, I would like to overview the past progress in our understanding of the DNA conformational behavior and, in particular, DNA condensation/compaction phenomenon and its relation to the DNA biological activity. This understanding was gained by designing relevant experimental models mimicking DNA behavior in the environment of living cell. Starting with a brief summary of classic experimental systems to study DNA condensation/compaction, in later parts, I highlight recent experimental methodologies to address the effects of macromolecular crowding and nanoscale and microscale confinements on DNA conformation dynamics. All the studies are discussed in the light of their relevance to DNA behavior in living cells, and future prospects of the field are outlined. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.cis.2016.02.005 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0001-8686 | |||||
| 書誌情報 |
en : Advances in Colloid and Interface Science 巻 232, p. 70-79, 発行日 2016-06 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
| URI | ||||||
| 識別子 | http://dx.doi.org/10.1016/j.cis.2016.02.005 | |||||
| 識別子タイプ | DOI | |||||
| URI | ||||||
| 識別子 | http://hdl.handle.net/2237/25148 | |||||
| 識別子タイプ | HDL | |||||
