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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

A cis-prenyltransferase from Methanosarcina acetivorans catalyzes both head-to-tail and nonhead-to-tail prenyl condensation

http://hdl.handle.net/2237/25160
http://hdl.handle.net/2237/25160
1fa6948a-a7ef-417b-be97-44ec82f098f7
名前 / ファイル ライセンス アクション
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Figure_11.pdf Figure_11.pdf ファイル公開:2017/06/01 (176.3 kB)
Figure_10.pdf Figure_10.pdf ファイル公開:2017/06/01 (166.1 kB)
Figure_9.pdf Figure_9.pdf ファイル公開:2017/06/01 (172.7 kB)
Figure_8.pdf Figure_8.pdf ファイル公開:2017/06/01 (71.6 kB)
Figure_7.pdf Figure_7.pdf ファイル公開:2017/06/01 (44.9 kB)
Figure_6.pdf Figure_6.pdf ファイル公開:2017/06/01 (149.9 kB)
Figure_5.pdf Figure_5.pdf ファイル公開:2017/06/01 (75.0 kB)
Figure_4.pdf Figure_4.pdf ファイル公開:2017/06/01 (137.8 kB)
Figure_3.pdf Figure_3.pdf ファイル公開:2017/06/01 (72.4 kB)
Figure_2.pdf Figure_2.pdf ファイル公開:2017/06/01 (183.5 kB)
Figure_1.pdf Figure_1.pdf ファイル公開:2017/06/01 (443.4 kB)
manuscript7_repository.pdf manuscript7_repository.pdf ファイル公開:2017/06/01 (271.3 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2016-12-08
タイトル
タイトル A cis-prenyltransferase from Methanosarcina acetivorans catalyzes both head-to-tail and nonhead-to-tail prenyl condensation
言語 en
著者 Ogawa, Takuya

× Ogawa, Takuya

WEKO 67869

en Ogawa, Takuya

Search repository
Emi, Koh-ichi

× Emi, Koh-ichi

WEKO 67870

en Emi, Koh-ichi

Search repository
Koga, Kazushi

× Koga, Kazushi

WEKO 67871

en Koga, Kazushi

Search repository
Yoshimura, Tohru

× Yoshimura, Tohru

WEKO 67872

en Yoshimura, Tohru

Search repository
Hemmi, Hisashi

× Hemmi, Hisashi

WEKO 67873

en Hemmi, Hisashi

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 This is the peer reviewed version of the following article: [Ogawa, T., Emi, K.-i., Koga, K., Yoshimura, T. and Hemmi, H. (2016), A cis-prenyltransferase from Methanosarcina acetivorans catalyzes both head-to-tail and nonhead-to-tail prenyl condensation. FEBS J, 283: 2369–2383], which has been published in final form at [http://doi.org/10.1111/febs.13749]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
キーワード
主題Scheme Other
主題 archaea
キーワード
主題Scheme Other
主題 cis-prenyltransferase
キーワード
主題Scheme Other
主題 diterpenoid
キーワード
主題Scheme Other
主題 glycosyl carrier lipid
キーワード
主題Scheme Other
主題 isoprenoid
抄録
内容記述 Cis-prenyltransferase usually consecutively catalyzes the head-to-tail condensation reactions of isopentenyl diphosphate to allylic prenyl diphosphate in the production of (E,Z-mixed) polyprenyl diphosphate, which is the precursor of glycosyl carrier lipids. Some recently discovered homologs of the enzyme, however, catalyze the nonhead-to-tail condensation reactions between allylic prenyl diphosphates. In this study, we characterize a cis-prenyltransferase homolog from a methanogenic archaeon, Methanosarcina acetivorans, to obtain information on the biosynthesis of the glycosyl carrier lipids within it. This enzyme catalyzes both head-to-tail and nonhead-to-tail condensation reactions. The kinetic analysis shows that the main reaction of the enzyme is consecutive head-to-tail prenyl condensation reactions yielding polyprenyl diphosphates, while the chain lengths of the major products seem shorter than expected for the precursor of glycosyl carrier lipids. On the other hand, a subsidiary reaction of the enzyme, i.e., nonhead-to-tail condensation between dimethylallyl diphosphate and farnesyl diphosphate, gives a novel diterpenoid compound, geranyllavandulyl diphosphate.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1111/febs.13749
ISSN
収録物識別子タイプ PISSN
収録物識別子 1742-464X
書誌情報 en : FEBS JOURNAL

巻 283, 号 12, p. 2369-2383, 発行日 2016-06
著者版フラグ
値 author
URI
識別子 http://doi.org/10.1111/febs.13749
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/25160
識別子タイプ HDL
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