{"created":"2021-03-01T06:31:03.353820+00:00","id":23218,"links":{},"metadata":{"_buckets":{"deposit":"40e231e8-6a71-4cf2-9e59-6bbcf7ff679d"},"_deposit":{"id":"23218","owners":[],"pid":{"revision_id":0,"type":"depid","value":"23218"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00023218","sets":["336:695:696"]},"author_link":["68798","68799","68800","68801","68802","68803"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"15","bibliographicPageEnd":"3802","bibliographicPageStart":"3794","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"Langmuir","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Liposomes encapsulating cytoskeletons have drawn much recent attention to develop an artificial cell-like chemical-machinery; however, as far as we know, there has been no report showing isothermally reversible morphological changes of liposomes containing cytoskeletons because the sets of various regulatory factors, that is, their interacting proteins, are required to control the state of every reaction system of cytoskeletons. Here we focused on hydrostatic pressure to control the polymerization state of microtubules (MTs) within cell-sized giant liposomes (diameters ∼10 μm). MT is the cytoskeleton formed by the polymerization of tubulin, and cytoskeletal systems consisting of MTs are very dynamic and play many important roles in living cells, such as the morphogenesis of nerve cells and formation of the spindle apparatus during mitosis. Using real-time imaging with a high-pressure microscope, we examined the effects of hydrostatic pressure on the morphology of tubulin-encapsulating giant liposomes. At ambient pressure (0.1 MPa), many liposomes formed protrusions due to tubulin polymerization within them. When high pressure (60 MPa) was applied, the protrusions shrank within several tens of seconds. This process was repeatedly inducible (around three times), and after the pressure was released, the protrusions regenerated within several minutes. These deformation rates of the liposomes are close to the velocities of migrating or shape-changing living cells rather than the shortening and elongation rates of the single MTs, which have been previously measured. These results demonstrate that the elongation and shortening of protrusions of giant liposomes is repeatedly controllable by regulating the polymerization state of MTs within them by applying and releasing hydrostatic pressure.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"http://doi.org/10.1021/acs.langmuir.6b00799"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/25411"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS Publications","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1021/acs.langmuir.6b00799","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Langmuir], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [http://pubsdc3.acs.org/articlesonrequest/AOR-pGncV5sJsY3y6M8YG8HV].”","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0743-7463","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Hayashi, Masahito","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68798","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishiyama, Masayoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68799","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kazayama, Yuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68800","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Toyota, Taro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68801","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Harada, Yoshie","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68802","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takiguchi, Kingo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"68803","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-03-29"}],"displaytype":"detail","filename":"LangmuirPaper.pdf","filesize":[{"value":"3.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"LangmuirPaper.pdf ファイル公開：2017/03/29","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/23218/files/LangmuirPaper.pdf"},"version_id":"12865f40-f48c-444b-a835-db0f58e22019"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Reversible Morphological Control of Tubulin-Encapsulating Giant Liposomes by Hydrostatic Pressure","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Reversible Morphological Control of Tubulin-Encapsulating Giant Liposomes by Hydrostatic Pressure","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["696"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-01-18"},"publish_date":"2017-01-18","publish_status":"0","recid":"23218","relation_version_is_last":true,"title":["Reversible Morphological Control of Tubulin-Encapsulating Giant Liposomes by Hydrostatic Pressure"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:12:52.447351+00:00"}