{"_buckets": {"deposit": "620a21bc-2ff4-4faf-88cf-c8aba248732a"}, "_deposit": {"id": "23231", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "23231"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00023231"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2013-06", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "6", "bibliographicPageEnd": "e66969", "bibliographicPageStart": "e66969", "bibliographicVolumeNumber": "8", "bibliographic_titles": [{"bibliographic_title": "PLOS ONE"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Biopolymers consist of three major classes, i.e., polynucleotides (DNA, RNA), polypeptides (proteins) and polysaccharides (sugar chains). It is widely accepted that polynucleotides and polypeptides play fundamental roles in the pathogenesis of neurodegenerative diseases. But, sugar chains have been poorly studied in this process, and their biological/clinical significance remains largely unexplored. Amyotrophic lateral sclerosis (ALS) is a motoneuron-degenerative disease, the pathogenesis of which requires both cell autonomous and non-cell autonomous processes. Here, we investigated the role of keratan sulfate (KS), a sulfated long sugar chain of proteoglycan, in ALS pathogenesis. We employed ALS model SOD1G93A mice and GlcNAc6ST-1\u2212/\u2212 mice, which are KS-deficient in the central nervous system. Unexpectedly, SOD1G93AGlcNAc6ST-1\u2212/\u2212 mice exhibited a significantly shorter lifespan than SOD1G93A mice and an accelerated appearance of clinical symptoms (body weight loss and decreased rotarod performance). KS expression was induced exclusively in a subpopulation of microglia in SOD1G93A mice, and became detectable around motoneurons in the ventral horn during the early disease phase before body weight loss. During this phase, the expression of M2 microglia markers was transiently enhanced in SOD1G93A mice, while this enhancement was attenuated in SOD1G93AGlcNAc6ST-1\u2212/\u2212 mice. Consistent with this, M2 microglia were markedly less during the early disease phase in SOD1G93AGlcNAc6ST-1\u2212/\u2212 mice. Moreover, KS expression in microglia was also detected in some human ALS cases. This study suggests that KS plays an indispensable, suppressive role in the early phase pathogenesis of ALS and may represent a new target for therapeutic intervention.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://dx.doi.org/10.1371/journal.pone.0066969"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/25426"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "PLOS"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "Copyright: (c) 2013 Hirano et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited."}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1932-6203", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hirano, Kenichi"}], "nameIdentifiers": [{"nameIdentifier": "68912", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohgomori, Tomohiro"}], "nameIdentifiers": [{"nameIdentifier": "68913", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kobayashi, Kazuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "68914", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tanaka, Fumiaki"}], "nameIdentifiers": [{"nameIdentifier": "68915", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsumoto, Tomohiro"}], "nameIdentifiers": [{"nameIdentifier": "68916", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Natori, Takamitsu"}], "nameIdentifiers": [{"nameIdentifier": "68917", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuyama, Yukihiro"}], "nameIdentifiers": [{"nameIdentifier": "68918", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Uchimura, Kenji"}], "nameIdentifiers": [{"nameIdentifier": "68919", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sakamoto, Kazuma"}], "nameIdentifiers": [{"nameIdentifier": "68920", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takeuchi, Hideyuki"}], "nameIdentifiers": [{"nameIdentifier": "68921", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirakawa, Akihiro"}], "nameIdentifiers": [{"nameIdentifier": "68922", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzumura, Akio"}], "nameIdentifiers": [{"nameIdentifier": "68923", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sobue, Gen"}], "nameIdentifiers": [{"nameIdentifier": "68924", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ishiguro, Naoki"}], "nameIdentifiers": [{"nameIdentifier": "68925", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Imagama, Shiro"}], "nameIdentifiers": [{"nameIdentifier": "68926", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kadomatsu, Kenji"}], "nameIdentifiers": [{"nameIdentifier": "68927", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-22"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "journal_pone_0066969.pdf", "filesize": [{"value": "15.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 15500000.0, "url": {"label": "journal_pone_0066969.pdf", "url": "https://nagoya.repo.nii.ac.jp/record/23231/files/journal_pone_0066969.pdf"}, "version_id": "4fd276c7-0981-419b-8bab-c6118d1abb6f"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS"}]}, "item_type_id": "10", "owner": "1", "path": ["499/500/501"], "permalink_uri": "http://hdl.handle.net/2237/25426", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2017-01-18"}, "publish_date": "2017-01-18", "publish_status": "0", "recid": "23231", "relation": {}, "relation_version_is_last": true, "title": ["Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS"], "weko_shared_id": null}