{"created":"2021-03-01T06:31:28.911771+00:00","id":23609,"links":{},"metadata":{"_buckets":{"deposit":"d4350cb9-bb66-42e2-9f9d-8052db84c2ae"},"_deposit":{"id":"23609","owners":[],"pid":{"revision_id":0,"type":"depid","value":"23609"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00023609","sets":["643:666:667"]},"author_link":["70138","70139","70140","70141"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"693","bibliographicPageStart":"689","bibliographicVolumeNumber":"122","bibliographic_titles":[{"bibliographic_title":"Journal of Bioscience and Bioengineering","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Escherichia coli YggS is a highly conserved pyridoxal 5′-phosphate (PLP)-binding protein whose biochemical function is currently unknown. A previous study with a yggS-deficient E. coli strain (ΔyggS) demonstrated that YggS controls l-Ile- and l-Val-metabolism by modulating 2-ketobutyrate (2-KB), l-2-aminobutyrate (l-2-AB), and/or coenzyme A (CoA) availability in a PLP-dependent fashion. In this study, we found that ΔyggS accumulates an unknown metabolite as judged by amino acid analyses. LC/MS and MS/MS analyses of the compound with propyl chloroformate derivatization, and co-chromatography analysis identified this compound as γ-l-glutamyl-l-2-aminobutyryl-glycine (ophthalmic acid), a glutathione (GSH) analogue in which the l-Cys moiety is replaced by l-2-AB. We also determine the metabolic consequence of the yggS mutation. Absence of YggS initially increases l-2-AB availability, and then causes ophthalmic acid accumulation and CoA limitation in the cell. The expression of a γ-glutamylcysteine synthetase and a glutathione synthetase in a ΔyggS background causes high-level accumulation of ophthalmic acid in the cells (∼1.2 nmol/mg cells) in a minimal synthetic medium. This opens the possibility of a first fermentative production of ophthalmic acid.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"http://dx.doi.org/10.1016/j.jbiosc.2016.06.010"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/25806"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jbiosc.2016.06.010","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1389-1723","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ito, Tomokazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"70138","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamauchi, Ayako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"70139","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hemmi, Hisashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"70140","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshimura, Tohru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"70141","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-01"}],"displaytype":"detail","filename":"yggs2_revise.pdf","filesize":[{"value":"694.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"yggs2_revise.pdf ファイル公開：2017/12/01","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/23609/files/yggs2_revise.pdf"},"version_id":"e0c9162e-77ce-4adf-b4a5-23ca791b61b1"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-01"}],"displaytype":"detail","filename":"YggS_second-revise.pdf","filesize":[{"value":"421.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"YggS_second-revise.pdf ファイル公開：2017/12/01","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/23609/files/YggS_second-revise.pdf"},"version_id":"055fdc0b-ab5d-43c4-b9c2-d4502c78457d"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Pyridoxal 5′-phosphate","subitem_subject_scheme":"Other"},{"subitem_subject":"Ophthalmic acid","subitem_subject_scheme":"Other"},{"subitem_subject":"YggS","subitem_subject_scheme":"Other"},{"subitem_subject":"Escherichia coli","subitem_subject_scheme":"Other"},{"subitem_subject":"Glutathione","subitem_subject_scheme":"Other"},{"subitem_subject":"Amino acid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-03-24"},"publish_date":"2017-03-24","publish_status":"0","recid":"23609","relation_version_is_last":true,"title":["Ophthalmic acid accumulation in an Escherichia coli mutant lacking the conserved pyridoxal 5′-phosphate-binding protein YggS"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:12:59.394408+00:00"}