{"_buckets": {"deposit": "1f2a468f-3fd5-401c-aac4-a145c8f65e16"}, "_deposit": {"id": "24073", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24073"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024073", "sets": ["667"]}, "author_link": ["71456", "71457", "71458", "71459", "71460"], "item_10_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "76", "bibliographicPageStart": "56", "bibliographicVolumeNumber": "284", "bibliographic_titles": [{"bibliographic_title": "FEBS Journal", "bibliographic_titleLang": "en"}]}]}, "item_10_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Apoptosis-linked gene 2 (ALG-2), which is a gene product of PDCD6, is a 22-kDa Ca2+-binding protein. Accumulating evidence points to a role for ALG-2 as a Ca2+-responsive adaptor protein. On binding to Ca2+, ALG-2 undergoes a conformational change that facilitates its interaction with various proteins. It also forms a homodimer and heterodimer with peflin, a paralog of ALG-2. However, the differences in cellular roles for the ALG-2 homodimer and ALG-2/peflin heterodimer are unclear. In the present study, we found that Trk-fused gene (TFG) protein interacted with the ALG-2 homodimer. Immunostaining analysis revealed that TFG and ALG-2 partially overlapped at endoplasmic reticulum exit sites (ERES), a platform for COPII-mediated protein transport from the endoplasmic reticulum. Time-lapse live-cell imaging demonstrated that both green fluorescent protein-fused TFG and mCherry-fused ALG-2 are recruited to ERES after thapsigargin treatment, which raises intracellular Ca2+ levels. Furthermore, overexpression of ALG-2 induced the accumulation of TFG at ERES. TFG has an ALG-2-binding motif and deletion of the motif decreased TFG binding to ALG-2 and shortened its half-life at ERES, suggesting a critical role for ALG-2 in retaining TFG at ERES. We also demonstrated, by in vitro cross-linking assays, that ALG-2 promoted the polymerization of TFG in a Ca2+-dependent manner. Collectively, the results suggest that ALG-2 acts as a Ca2+-sensitive adaptor to concentrate and polymerize TFG at ERES, supporting a potential role for ALG-2 in COPII-dependent trafficking from the endoplasmic reticulum.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1111/febs.13949"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/26285"}]}, "item_10_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Wiley", "subitem_publisher_language": "en"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1111/febs.13949", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "This is the peer reviewed version of the following article: [Kanadome, T., Shibata, H., Kuwata, K., Takahara, T. and Maki, M. (2017), The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein. FEBS J, 284: 56–76. doi:10.1111/febs.13949], which has been published in final form at [http://doi.org/10.1111/febs.13949]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.", "subitem_rights_language": "en"}]}, "item_10_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1742-464X", "subitem_source_identifier_type": "PISSN"}]}, "item_1615787544753": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_ab4af688f83e57aa", "subitem_version_type": "AM"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kanadome, Takashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "71456", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shibata, Hideki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "71457", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kuwata, Keiko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "71458", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Takahara, Terunao", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "71459", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Maki, Masatoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "71460", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-01-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "FJ_KanadomeT_ShibataH_170510.pdf", "filesize": [{"value": "7.5 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 7500000.0, "url": {"label": "FJ_KanadomeT_ShibataH_170510.pdf ファイル公開：2018/01/01", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/24073/files/FJ_KanadomeT_ShibataH_170510.pdf"}, "version_id": "c9e86cc8-c81f-46d2-9152-96e7fbb6ed9f"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein", "subitem_title_language": "en"}]}, "item_type_id": "10", "owner": "1", "path": ["667"], "permalink_uri": "http://hdl.handle.net/2237/26285", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2017-05-11"}, "publish_date": "2017-05-11", "publish_status": "0", "recid": "24073", "relation": {}, "relation_version_is_last": true, "title": ["The calcium-binding protein ALG-2 promotes endoplasmic reticulum exit site localization and polymerization of Trk-fused gene (TFG) protein"], "weko_shared_id": -1}