{"_buckets": {"deposit": "9e7dd393-6e80-4346-9307-56ca814cc852"}, "_deposit": {"id": "24372", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "24372"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00024372"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-02-26", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "117", "bibliographicPageStart": "113", "bibliographicVolumeNumber": "484", "bibliographic_titles": [{"bibliographic_title": "Biochemical and Biophysical Research Communications"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Epstein-Barr virus (EBV) nuclear antigen 1 (EBNA1) is expressed in every EBV-positive tumor and is essential for the maintenance, replication, and transcription of the EBV genome in the nucleus of host cells. EBNA1 is a serine phosphoprotein, and it has been shown that phosphorylation of S385 in the nuclear localization signal (NLS) of EBNA1 increases the binding affinity to the nuclear import adaptor importin-\u03b11 as well as importin-\u03b15, and stimulates nuclear import of EBNA1. To gain insights into how phosphorylation of the EBNA1 NLS regulates nuclear import, we have determined the crystal structures of two peptide complexes of importin-\u03b11: one with S385-phosphorylated EBNA1 NLS peptide, determined at 2.0 \u00c5 resolution, and one with non-phosphorylated EBNA1 NLS peptide, determined at 2.2 \u00c5 resolution. The structures show that EBNA1 NLS binds to the major and minor NLS-binding sites of importin-\u03b11, and indicate that the binding affinity of the EBNA1 NLS to the minor NLS-binding site could be enhanced by phosphorylation of S385 through electrostatic interaction between the phosphate group of phospho-S385 and K392 of importin-\u03b11 (corresponding to R395 of importin-\u03b15) on armadillo repeat 8.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "https://doi.org/10.1016/j.bbrc.2017.01.063"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/26586"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0006-291X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Nakada, Ryohei"}], "nameIdentifiers": [{"nameIdentifier": "72193", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hirano, Hidemi"}], "nameIdentifiers": [{"nameIdentifier": "72194", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsuura, Yoshiyuki"}], "nameIdentifiers": [{"nameIdentifier": "72195", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-26"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Nakada2017BBRC_for_depository.pdf", "filesize": [{"value": "2.7 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 2700000.0, "url": {"label": "Nakada2017BBRC_for_depository.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2018/02/26", "url": "https://nagoya.repo.nii.ac.jp/record/24372/files/Nakada2017BBRC_for_depository.pdf"}, "version_id": "65a75bfa-100e-4e46-b04f-4ece2e635c10"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Structural basis for the regulation of nuclear import of Epstein-Barr virus nuclear antigen 1 (EBNA1) by phosphorylation of the nuclear localization signal", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Structural basis for the regulation of nuclear import of Epstein-Barr virus nuclear antigen 1 (EBNA1) by phosphorylation of the nuclear localization signal"}]}, "item_type_id": "10", "owner": "1", "path": ["336/695/696"], "permalink_uri": "http://hdl.handle.net/2237/26586", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2017-06-07"}, "publish_date": "2017-06-07", "publish_status": "0", "recid": "24372", "relation": {}, "relation_version_is_last": true, "title": ["Structural basis for the regulation of nuclear import of Epstein-Barr virus nuclear antigen 1 (EBNA1) by phosphorylation of the nuclear localization signal"], "weko_shared_id": 3}