{"created":"2021-03-01T06:32:19.942492+00:00","id":24389,"links":{},"metadata":{"_buckets":{"deposit":"625f09d3-3540-4357-890e-930ed60c46f3"},"_deposit":{"id":"24389","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24389"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00024389","sets":["643:666:667"]},"author_link":["72239","72240","72241","72242","72243","72244","72245","72246"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-02-10","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"2469","bibliographicPageStart":"2457","bibliographicVolumeNumber":"292","bibliographic_titles":[{"bibliographic_title":"The Journal of Biological Chemistry","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The biosynthesis of isopentenyl diphosphate, a fundamental precursor for isoprenoids, via the mevalonate pathway is completed by diphosphomevalonate decarboxylase. This enzyme catalyzes the formation of isopentenyl diphosphate through the ATP-dependent phosphorylation of the 3-hydroxyl group of (R)-5-diphosphomevalonate followed by decarboxylation coupled with the elimination of the 3-phosphate group. In this reaction, a conserved aspartate residue has been proposed to be involved in the phosphorylation step as the general base catalyst that abstracts a proton from the 3-hydroxyl group. In this study, the catalytic mechanism of this rare type of decarboxylase is re-investigated by structural and mutagenic studies on the enzyme from a thermoacidophilic archaeon Sulfolobus solfataricus. The crystal structures of the archaeal enzyme in complex with (R)-5-diphosphomevalonate and adenosine 5′-O-(3-thio)triphosphate or with (R)-5-diphosphomevalonate and ADP are newly solved, and theoretical analysis based on the structure suggests the inability of proton abstraction by the conserved aspartate residue, Asp-281. Site-directed mutagenesis on Asp-281 creates mutants that only show diphosphomevalonate 3-kinase activity, demonstrating that the residue is required in the process of phosphate elimination/decarboxylation, rather than in the preceding phosphorylation step. These results enable discussion of the catalytic roles of the aspartate residue and provide clear proof of the involvement of a long predicted intermediate, (R)-3-phospho-5-diphosphomevalonate, in the reaction of the enzyme.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"http://doi.org/10.1074/jbc.M116.752535"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/26599"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"American Society for Biochemistry and Molecular Biology","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1074/jbc.M116.752535","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This research was originally published in The Journal of Biological Chemistry. Kento Motoyama, Hideaki Unno, Ai Hattori, Tomohiro Takaoka, Hiroshi Ishikita, Hiroshi Kawaide, Tohru Yoshimura and Hisashi Hemmi. A Single Amino Acid Mutation Converts (R)-5-Diphosphomevalonate Decarboxylase into a Kinase. The Journal of Biological Chemistry. 2017; 292:pp.2457-pp.2469. © the American Society for Biochemistry and Molecular Biology.","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0021-9258","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Motoyama, Kento","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72239","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Unno, Hideaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72240","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hattori, Ai","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72241","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takaoka, Tomohiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72242","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishikita, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72243","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawaide, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72244","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshimura, Tohru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72245","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hemmi, Hisashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72246","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-02-10"}],"displaytype":"detail","filename":"J_Biol_Chem-2017-Motoyama-2457-69.pdf","filesize":[{"value":"2.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"J_Biol_Chem-2017-Motoyama-2457-69.pdf ファイル公開：2018/02/10","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/24389/files/J_Biol_Chem-2017-Motoyama-2457-69.pdf"},"version_id":"f12566b0-f262-4a69-8c2c-da1984fd06f1"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A Single Amino Acid Mutation Converts (R)-5-Diphosphomevalonate Decarboxylase into a Kinase","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A Single Amino Acid Mutation Converts (R)-5-Diphosphomevalonate Decarboxylase into a Kinase","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-06-08"},"publish_date":"2017-06-08","publish_status":"0","recid":"24389","relation_version_is_last":true,"title":["A Single Amino Acid Mutation Converts (R)-5-Diphosphomevalonate Decarboxylase into a Kinase"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:54:43.918942+00:00"}