{"created":"2021-03-01T06:32:20.653077+00:00","id":24400,"links":{},"metadata":{"_buckets":{"deposit":"2a3d8eac-b653-41bf-be1e-8e7cf8b92586"},"_deposit":{"id":"24400","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24400"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00024400","sets":["1903:1904:1905"]},"author_link":["72295","72296","72297","72298","72299","72300"],"control_number":"24400","item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"474","bibliographicPageStart":"469","bibliographicVolumeNumber":"81","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transglutaminase is an enzyme family responsible for post-translational modification such as protein cross-linking and the attachment of primary amine and/or deamidation of glutamine-residue in proteins. Medaka (Oryzias latipes), a recently established model fish, has similar functional proteins to those characterized in mammals. Previously, we found the apparent orthologues that correspond to human transglutaminases in medaka. In this study, regarding the medaka orthologue of human tissue-type transglutaminase (OlTGT), recombinant protein was expressed in an active form in bacteria cultured at low temperature. Using the recombinant protein, we biochemically characterized the enzymatic activity and also obtained a monoclonal antibody that specifically recognized OlTGT. Immunochemical analysis revealed that OlTGT was not expressed ubiquitously, unlike its mammalian orthologue, but in primarily limited tissues such as the eye, brain, spinal cord, and gas gland.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/26612"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Taylor & Francis","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1080/09168451.2016.1256757","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"(c)日本農芸化学会. This is an Accepted Manuscript of an article published by Taylor & Francis Group in Bioscience, Biotechnology, and Biochemistry on 18/11/2016, available online: http://www.tandfonline.com/10.1080/09168451.2016.1256757.","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0916-8451","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Takada, Yuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72295","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Watanabe, Yuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72296","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okuya, Kazuho","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72297","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tatsukawa, Hideki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72298","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hashimoto, Hisashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72299","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hitomi, Kiyotaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72300","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-01-01"}],"displaytype":"detail","filename":"Hitomi_BBB_Takada_Nagoya_Univ.pdf","filesize":[{"value":"1.5 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Hitomi_BBB_Takada_Nagoya_Univ.pdf ファイル公開：2018/01/01","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/24400/files/Hitomi_BBB_Takada_Nagoya_Univ.pdf"},"version_id":"7e521b2d-109e-4897-991e-1b7595b0fb64"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2)","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["1905"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-06-09"},"publish_date":"2017-06-09","publish_status":"0","recid":"24400","relation_version_is_last":true,"title":["Biochemical characterization of the medaka (Oryzias latipes) orthologue for mammalian tissue-type transglutaminase (TG2)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T05:03:49.057498+00:00"}