{"created":"2021-03-01T06:32:22.276802+00:00","id":24425,"links":{},"metadata":{"_buckets":{"deposit":"75d00fb1-05b6-411b-a86b-05bc64b5296c"},"_deposit":{"id":"24425","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24425"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00024425","sets":["499:500:501"]},"author_link":["72359","72360","72361","72362","72363","72364"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-09-23","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"1235","bibliographicPageStart":"1230","bibliographicVolumeNumber":"478","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Although excessive mechanical stress loading is known to induce articular cartilage degradation, the mechanism underlying this process is unclear. The interaction between hyaluronan (HA) and its primary receptor CD44 maintains the homeostasis of articular chondrocytes. CD44 cleavage and the generation of CD44-intracellular domain (ICD) can lead to the loss of extracellular matrices in chondrocytes. Here we studied the effects of cyclic tensile strain (CTS) loading, a representative mechanical stress, on CD44 cleavage. CTS loading (1 Hz and 20% elongation for 48 h) increased ADAM10 expression and CD44 cleavage in HCS-2/8 cells, a human chondrocytic cell line. Co-treatment with a chemical ADAM10 inhibitor significantly suppressed CTS loading-induced CD44 cleavage. Chemical inhibition of transient receptor potential vanilloid 4 (TRPV4) significantly suppressed CTS loading-induced ADAM10 expression and CD44 cleavage. Conversely, chemical activation of TRPV4 increased ADAM10 expression and enhanced CD44 cleavage. Our findings suggest that CTS loading significantly increases the expression of ADAM10, which in turn enhances CD44 cleavage in HCS-2/8 cells. The primary mechanoreceptor mediating this process is TRPV4. This signature event could provide an avenue for intervention in the prevention of cartilage degradation leading to OA.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1016/j.bbrc.2016.08.099"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/26633"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2016.08.099","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kobayakawa, Tomonori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72359","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Takahashi, Nobunori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72360","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sobue, Yasumori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72361","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Terabe, Kenya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72362","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ishiguro, Naoki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72363","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kojima, Toshihisa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72364","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-09-23"}],"displaytype":"detail","filename":"Kobayakawa_BBRC_manuscript_final_version.pdf","filesize":[{"value":"400.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Kobayakawa_BBRC_manuscript_final_version.pdf ファイル公開：2017/09/23","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/24425/files/Kobayakawa_BBRC_manuscript_final_version.pdf"},"version_id":"15143c85-c0b0-4256-93a5-b55269c8510b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Mechanical loading","subitem_subject_scheme":"Other"},{"subitem_subject":"CD44 cleavage","subitem_subject_scheme":"Other"},{"subitem_subject":"ADAM-10","subitem_subject_scheme":"Other"},{"subitem_subject":"TRPV4","subitem_subject_scheme":"Other"},{"subitem_subject":"HCS-2/8","subitem_subject_scheme":"Other"},{"subitem_subject":"Osteoarthritis","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Mechanical stress loading induces CD44 cleavage in human chondrocytic HCS-2/8 cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Mechanical stress loading induces CD44 cleavage in human chondrocytic HCS-2/8 cells","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["501"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-06-09"},"publish_date":"2017-06-09","publish_status":"0","recid":"24425","relation_version_is_last":true,"title":["Mechanical stress loading induces CD44 cleavage in human chondrocytic HCS-2/8 cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:45:35.244890+00:00"}