{"created":"2021-03-01T06:32:24.630702+00:00","id":24461,"links":{},"metadata":{"_buckets":{"deposit":"e087b6c9-3de8-484b-be5e-9df32f0b3736"},"_deposit":{"id":"24461","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24461"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00024461","sets":["499:500:501"]},"author_link":["72463","72464","72465","72466","72467","72468","72469","72470","72471"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"755","bibliographicPageStart":"740","bibliographicVolumeNumber":"65","bibliographic_titles":[{"bibliographic_title":"GLIA","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Under a quiescent state, microglia exhibit a ramified shape, rather than the amoeboid-like morphology following injury or inflammation. The manipulation of microglial morphology in vitro has not been very successful, which has impeded the progress of microglial studies. We demonstrate that lysophosphatidylserine (LysoPS), a kind of lysophospholipids, rapidly and substantially alters the morphology of primary cultured microglia to an in vivo-like ramified shape in a receptor independent manner. This mechanism is mediated by Cdc42 activity. LysoPS is incorporated into the plasma membrane and converted to phosphatidylserine (PS) via the Lands' cycle. The accumulated PS on the membrane recruits Cdc42. Both Cdc42 and PS colocalize predominantly in primary and secondary processes, but not in peripheral branches or tips of microglia. Along with the morphological changes LysoPS suppresses inflammatory cytokine production and NF-kB activity. The present study provides a tool to manipulate a microglial phenotype from an amoeboid to a fully ramified in vitro, which certainly contributes to studies exploring microglial physiology and pathology.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"http://doi.org/10.1002/glia.23123"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/26657"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/glia.23123","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is the peer reviewed version of the following article: [Tokizane K, Konishi H, Makide K, et al. Phospholipid localization implies microglial morphology and function via Cdc42 in vitro. Glia. 2017;65:740–755. https://doi.org/10.1002/glia.23123], which has been published in final form at [http://doi.org/10.1002/glia.23123]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0894-1491","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tokizane, Kyohei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72463","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Konishi, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72464","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Makide, Kumiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72465","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kawana, Hiroki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72466","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakamuta, Shinichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72467","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kaibuchi, Kozo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72468","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohwada, Tomohiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72469","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Aoki, Junken","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72470","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kiyama, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72471","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-01"}],"displaytype":"detail","filename":"Glia.pdf","filesize":[{"value":"6.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Glia.pdf ファイル公開：2018/05/01","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/24461/files/Glia.pdf"},"version_id":"19315534-f4de-4160-affc-2e774913297b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Phospholipid localization implies microglial morphology and function via Cdc42 in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Phospholipid localization implies microglial morphology and function via Cdc42 in vitro","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["501"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-06-13"},"publish_date":"2017-06-13","publish_status":"0","recid":"24461","relation_version_is_last":true,"title":["Phospholipid localization implies microglial morphology and function via Cdc42 in vitro"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:14:48.086571+00:00"}