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  1. D400 創薬科学研究科
  2. D400a 雑誌掲載論文
  3. 学術雑誌

Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast

http://hdl.handle.net/2237/26771
32783fa9-af5f-4097-a2ee-268b5639c5ce
名前 / ファイル ライセンス アクション
MGG.pdf MGG.pdf ファイル公開:2018/04/01 (394.2 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2017-07-05
タイトル
タイトル Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast
著者 Shimasaki, Takafumi

× Shimasaki, Takafumi

WEKO 72678

Shimasaki, Takafumi

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Ohtsuka, Hokuto

× Ohtsuka, Hokuto

WEKO 72679

Ohtsuka, Hokuto

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Naito, Chikako

× Naito, Chikako

WEKO 72680

Naito, Chikako

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Azuma, Kenko

× Azuma, Kenko

WEKO 72681

Azuma, Kenko

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Tenno, Takeshi

× Tenno, Takeshi

WEKO 72682

Tenno, Takeshi

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Hiroaki, Hidekazu

× Hiroaki, Hidekazu

WEKO 72683

Hiroaki, Hidekazu

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Murakami, Hiroshi

× Murakami, Hiroshi

WEKO 72684

Murakami, Hiroshi

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Aiba, Hirofumi

× Aiba, Hirofumi

WEKO 72685

Aiba, Hirofumi

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権利
権利情報 The final publication is available at Springer via http://doi.org/10.1007/s00438-016-1285-x
抄録
内容記述 Overexpression of Ecl1-family genes (ecl1+, ecl2+, and ecl3+) results in the extension of the chronological life span in Schizosaccharomyces pombe. However, the mechanism for this extension has not been defined clearly. Ecl1-family proteins consist of approximately 80 amino acids, and four cysteine residues are conserved in their N-terminal domains. This study focused on the Ecl1 protein, mutating its cysteine residues sequentially to confirm their importance. As a result, all mutated Ecl1 proteins nearly lost the function to extend the chronological life span, suggesting that these four cysteine residues are essential for the Ecl1 protein. Utilizing ICP-AES (inductively coupled plasma atomic emission spectroscopy) analysis, we found that wild-type Ecl1 proteins contain zinc, while cysteine-mutated Ecl1 proteins do not. We also analyzed the effect of environmental zinc on the chronological life span. We found that zinc limitation extends the chronological life span, and this extension depends on the Ecl1-family proteins.
内容記述タイプ Abstract
出版者
出版者 Springer
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
ISSN
収録物識別子タイプ ISSN
収録物識別子 1617-4615
書誌情報 Molecular Genetics and Genomics

巻 292, 号 2, p. 475-481, 発行日 2017-04
著者版フラグ
値 author
URI
識別子 http://doi.org/10.1007/s00438-016-1285-x
識別子タイプ DOI
URI
識別子 http://hdl.handle.net/2237/26771
識別子タイプ HDL
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