{"created":"2021-03-01T06:32:36.221960+00:00","id":24638,"links":{},"metadata":{"_buckets":{"deposit":"c6387840-9c23-4b8a-a243-b4c7e1dd4c71"},"_deposit":{"id":"24638","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24638"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00024638","sets":["329:330:331"]},"author_link":["72952","72953","72954","72955","72956","72957"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-05-02","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"18","bibliographicPageEnd":"E3708","bibliographicPageStart":"E3699","bibliographicVolumeNumber":"114","bibliographic_titles":[{"bibliographic_title":"Proceedings of the National Academy of Sciences of the United States of America","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The temporal order of physiology and behavior in mammals is primarily regulated by the circadian pacemaker located in the hypothalamic suprachiasmatic nucleus (SCN). Taking advantage of bioluminescence reporters, we monitored the circadian rhythms of the expression of clock genes Per1 and Bmal1 in the SCN of freely moving mice and found that the rate of phase shifts induced by a single light pulse was different in the two rhythms. The Per1-luc rhythm was phase-delayed instantaneously by the light presented at the subjective evening in parallel with the activity onset of behavioral rhythm, whereas the Bmal1-ELuc rhythm was phase-delayed gradually, similar to the activity offset. The dissociation was confirmed in cultured SCN slices of mice carrying both Per1-luc and Bmal1-ELuc reporters. The two rhythms in a single SCN slice showed significantly different periods in a long-term (3 wk) culture and were internally desynchronized. Regional specificity in the SCN was not detected for the period of Per1-luc and Bmal1-ELuc rhythms. Furthermore, neither is synchronized with circadian intracellular Ca^2+ rhythms monitored by a calcium indicator, GCaMP6s, or with firing rhythms monitored on a multielectrode array dish, although the coupling between the circadian firing and Ca^2+ rhythms persisted during culture. These findings indicate that the expressions of two key clock genes, Per1 and Bmal1, in the SCN are regulated in such a way that they may adopt different phases and free-running periods relative to each other and are respectively associated with the expression of activity onset and offset.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"http://doi.org/10.1073/pnas.1613374114"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/26856"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"National Academy of Sciences of the United States of America","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://doi.org/10.1073/pnas.1613374114","subitem_relation_type_select":"DOI"}}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1091-6490","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ono, Daisuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72952","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Honma, Sato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72953","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakajima, Yoshihiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72954","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kuroda, Shigeru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72955","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Enoki, Ryosuke","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72956","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Honma, Ken-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"72957","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-05-02"}],"displaytype":"detail","filename":"20170324_Ono.pdf","filesize":[{"value":"1.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"20170324_Ono.pdf ファイル公開：2018/05/02","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/24638/files/20170324_Ono.pdf"},"version_id":"086f3a1c-6a89-468d-9724-1640a1f20a21"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"clock gene","subitem_subject_scheme":"Other"},{"subitem_subject":"in vivo recording","subitem_subject_scheme":"Other"},{"subitem_subject":"suprachiasmatic nucleus","subitem_subject_scheme":"Other"},{"subitem_subject":"photic phase resetting","subitem_subject_scheme":"Other"},{"subitem_subject":"E and M oscillators","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Dissociation of Per1 and Bmal1 circadian rhythms in the suprachiasmatic nucleus in parallel with behavioral outputs","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Dissociation of Per1 and Bmal1 circadian rhythms in the suprachiasmatic nucleus in parallel with behavioral outputs","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["331"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-08-01"},"publish_date":"2017-08-01","publish_status":"0","recid":"24638","relation_version_is_last":true,"title":["Dissociation of Per1 and Bmal1 circadian rhythms in the suprachiasmatic nucleus in parallel with behavioral outputs"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T05:03:29.504224+00:00"}