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c-Rel Promotes Invasion of Choriocarcinoma Cells via PI3K/AKT Signaling
http://hdl.handle.net/2237/26939
http://hdl.handle.net/2237/26939e28b5ed8-601e-471f-ba31-0f28a922c056
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2017-09-04 | |||||
タイトル | ||||||
タイトル | c-Rel Promotes Invasion of Choriocarcinoma Cells via PI3K/AKT Signaling | |||||
言語 | en | |||||
著者 |
Sekiya, Yoko
× Sekiya, Yoko× Yamamoto, Eiko× Niimi, Kaoru× Nishino, Kimihiro× Nakamura, Kenichi× Kotani, Tomomi× Kajiyama, Hiroaki× Shibata, Kiyosumi× Kikkawa, Fumitaka |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | ‘This is the peer-reviewed but unedited manuscript version of the following article: [Oncology. v.92, n.5, 2017, p.299-310 (DOI: 10.1159/000458529)]. The final, published version is available at http://www.karger.com/?doi=10.1159/000458529.’ | |||||
抄録 | ||||||
内容記述 | Objective: Choriocarcinoma is the most common epithelial cancer among gestational trophoblastic diseases (GTDs); the mechanism of trophoblastic carcinogenesis is unknown. This study aimed to examine the expression of NF-κB family proteins in GTDs and placental tissues as well as the roles of c-Rel in choriocarcinoma. Methods: We examined the expression of NF-κB family proteins in normal placenta and hydatidiform mole tissues as well as extravillous trophoblast (EVT) and choriocarcinoma cell lines by Western blot and immunohistochemistry. Immunoprecipitation was performed to determine which proteins can bind with c-Rel in choriocarcinoma cells. To investigate the roles of c-Rel in choriocarcinoma, we examined the effects of c-Rel knockdown and overexpression on cell proliferation, migration, and invasion using small interfering RNAs and gene activation plasmid. Results: The expression of c-Rel was strong in choriocarcinoma and EVTs, but very weak in villi of normal placenta and hydatidiform mole. Immunoprecipitation suggested that c-Rel heterodimerizes with p65 in choriocarcinoma. c-Rel knockdown reduced invasion, migration, and AKT phosphorylation in choriocarcinoma cells. c-Rel overexpression in choriocarcinoma increased migratory and invasive abilities, and the effect on invasion was inhibited by a PI3K inhibitor. Conclusion: These findings suggest that c-Rel might play a role in promoting the invasion of choriocarcinoma cells through PI3K/AKT signaling. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Karger | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1159/000458529 | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 0030-2414 | |||||
書誌情報 |
en : Oncology 巻 92, 号 5, p. 299-310, 発行日 2017-05 |
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著者版フラグ | ||||||
値 | author | |||||
URI | ||||||
識別子 | https://doi.org/10.1159/000458529 | |||||
識別子タイプ | DOI | |||||
URI | ||||||
識別子 | http://hdl.handle.net/2237/26939 | |||||
識別子タイプ | HDL |