{"created":"2021-03-01T06:32:43.434156+00:00","id":24747,"links":{},"metadata":{"_buckets":{"deposit":"9d699af9-d028-4406-8e34-a74c7f5f2dee"},"_deposit":{"id":"24747","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24747"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00024747","sets":["499:500:501"]},"author_link":["73506","73507","73508","73509","73510","73511","73512","73513","73514","73515","73516"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-07","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"917","bibliographicPageStart":"904","bibliographicVolumeNumber":"108","bibliographic_titles":[{"bibliographic_title":"Free Radical Biology and Medicine","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Non-thermal plasma (NTP) is a potential new therapeutic modality for cancer. However, its mechanism of action remains unclear. Herein, we studied the effect of NTP on mesothelioma cells and fibroblasts to understand its anti-proliferative efficacy. Interestingly, NTP demonstrated greater selective anti-proliferative activity against mesothelioma cells relative to fibroblasts than cisplatin, which is used for mesothelioma treatment. The anti-proliferative effect of NTP was enhanced by pre-incubation with the cellular iron donor, ferric ammonium citrate (FAC), and inhibited by iron chelation using desferrioxamine (DFO). Three oxidative stress probes (CM-H2DCFDA, MitoSOX and C11-BODIPY) demonstrated reactive oxygen species (ROS) generation by NTP, which was inhibited by DFO. Moreover, NTP decreased transferrin receptor-1 and increased ferritin-H and -L chain expression that was correlated with decreased iron-regulatory protein expression and RNA-binding activity. This regulation was potentially due to increased intracellular iron in lysosomes, which was demonstrated via the Fe(II)-selective probe, HMRhoNox-M, and was consistent with autophagic-induction. Immunofluorescence using LysoTracker and Pepstatin A probes demonstrated increased cellular lysosome content, which was confirmed by elevated LAMP1 expression. The enhanced lysosomal biogenesis after NTP could be due to the observed increase in fluid-phase endocytosis and early endosome formation. These results suggest NTP acts as a stressor, which results in increased endocytosis, lysosome content and autophagy. In fact, NTP rapidly increased autophagosome formation, as judged by increased LC3B-II expression, which co-localized with LAMP1, indicating autophagolysosome formation. Autophagic-induction by NTP was confirmed using electron microscopy. In summary, NTP acts as a cellular stressor to rapidly induce fluid-phase endocytosis, lysosome biogenesis and autophagy.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"https://doi.org/10.1016/j.freeradbiomed.2017.04.368"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/26969"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.freeradbiomed.2017.04.368","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0891-5849","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shi, Lei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73506","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ito, Fumiya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73507","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Wang, Yue","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73508","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okazaki, Yasumasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73509","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Hiromasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73510","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mizuno, Masaaki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73511","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hori, Masaru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73512","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hirayama, Tasuku","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73513","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagasawa, Hideko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73514","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Richardson, Des R.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73515","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Toyokuni, Shinya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"73516","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-07-01"}],"displaytype":"detail","filename":"Lei_Shi_FRBM_Plasma_2017_Repository.pdf","filesize":[{"value":"5.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Lei_Shi_FRBM_Plasma_2017_Repository.pdf ファイル公開：2018/07/01","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/24747/files/Lei_Shi_FRBM_Plasma_2017_Repository.pdf"},"version_id":"7f4c8553-65f3-47ff-9f18-c4f2d94ab905"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["501"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-09-06"},"publish_date":"2017-09-06","publish_status":"0","recid":"24747","relation_version_is_last":true,"title":["Non-thermal plasma induces a stress response in mesothelioma cells resulting in increased endocytosis, lysosome biogenesis and autophagy"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:14:57.500120+00:00"}