{"created":"2021-03-01T06:32:55.545790+00:00","id":24930,"links":{},"metadata":{"_buckets":{"deposit":"ba042d97-c9d9-45d7-8f58-b8dba972b3ce"},"_deposit":{"id":"24930","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24930"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00024930","sets":["499:500:501"]},"author_link":["74266","74267","74268","74269","74270","74271","74272","74273","74274","74275"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"4","bibliographicPageEnd":"670","bibliographicPageStart":"661","bibliographicVolumeNumber":"32","bibliographic_titles":[{"bibliographic_title":"Neurotoxicity Research","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Previous studies showed that overexposure to manganese causes parkinsonism, a disorder of dopaminergic neurons. Previous studies also showed that activity of c-RET kinase controls dopamine production through regulation of tyrosine hydroxylase (TH) expression, suggesting the involvement of c-RET in the development of parkinsonism. To our knowledge, however, there is no report showing a correlation between manganese-mediated parkinsonism and c-RET. In this study, we examined the effect of manganese on the expression and/or activation levels of c-RET and TH in human TH-expressing cells (TGW cells). We first found that treatment with 30 and 100 μM manganese resulted in reduction of c-RET transcript level and degradation of c-RET protein through promotion of ubiquitination. We then examined the biological significance of manganese-mediated decrease of c-RET protein expression. Decreased TH expression with decreased c-RET kinase activity was observed in c-RET protein-depleted TGW cells by treatment with manganese (30 μM) as well as by c-RET siRNA transfection. Since TH protein has been shown to be involved in the dopamine-producing pathway in previous studies, our results indicate the possibility that manganese-mediated reduction of TH expression and phosphorylation via decreased expression of c-RET protein in neural cells is involved in parkinsonism induced by manganese.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"http://doi.org/10.1007/s12640-017-9783-0"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/27152"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s12640-017-9783-0","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"The final publication is available at Springer via http://doi.org/10.1007/s12640-017-9783-0","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1029-8428","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kumasaka, Mayuko Y.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74266","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yajima, Ichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74267","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohgami, Nobutaka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74268","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ninomiya, Hiromasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74269","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Iida, Machiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74270","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Li, Xiang","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74271","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oshino, Reina","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanihata, Hiroko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yoshinaga, Masafumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74274","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kato, Masashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74275","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-11-01"}],"displaytype":"detail","filename":"Kumasaka_et_al.pdf","filesize":[{"value":"2.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Kumasaka_et_al.pdf ファイル公開日:2018/11/01","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/24930/files/Kumasaka_et_al.pdf"},"version_id":"864bf2c5-3417-4d47-a303-35b37892b2f0"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Manganese","subitem_subject_scheme":"Other"},{"subitem_subject":"Metals","subitem_subject_scheme":"Other"},{"subitem_subject":"Protein expression","subitem_subject_scheme":"Other"},{"subitem_subject":"RET","subitem_subject_scheme":"Other"},{"subitem_subject":"Tyrosine kinase","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Manganese-mediated decrease in levels of c-RET and tyrosine hydroxylase expression in vitro","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Manganese-mediated decrease in levels of c-RET and tyrosine hydroxylase expression in vitro","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["501"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-12-07"},"publish_date":"2017-12-07","publish_status":"0","recid":"24930","relation_version_is_last":true,"title":["Manganese-mediated decrease in levels of c-RET and tyrosine hydroxylase expression in vitro"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:14:03.745965+00:00"}