{"created":"2021-03-01T06:32:56.878090+00:00","id":24950,"links":{},"metadata":{"_buckets":{"deposit":"bcf7aeab-4e4e-4a89-a15f-f0153f6de665"},"_deposit":{"id":"24950","owners":[],"pid":{"revision_id":0,"type":"depid","value":"24950"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00024950","sets":["336:695:696"]},"author_link":["74381","74382"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-09-23","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"613","bibliographicPageStart":"609","bibliographicVolumeNumber":"491","bibliographic_titles":[{"bibliographic_title":"BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ran-binding protein 3 (RanBP3) is a primarily nuclear Ran-binding protein that functions as an accessory factor in the Ran GTPase system. RanBP3 associates with Ran-specific nucleotide exchange factor RCC1 and enhances its catalytic activity towards Ran. RanBP3 also promotes CRM1-mediated nuclear export as well as CRM1-independent nuclear export of β-catenin, Smad2, and Smad3. Nuclear import of RanBP3 is dependent on the nuclear import adaptor protein importin-α and, RanBP3 is imported more efficiently by importin-α3 than by other members of the importin-α family. Protein kinase signaling pathways control nucleocytoplasmic transport through phosphorylation of RanBP3 at Ser58, immediately C-terminal to the nuclear localization signal (NLS) in the N-terminal region of RanBP3. Here we report the crystal structure of human importin-α3 bound to an N-terminal fragment of human RanBP3 containing the NLS sequence that is necessary and sufficient for nuclear import. The structure reveals that RanBP3 binds to importin-α3 residues that are strictly conserved in all seven isoforms of human importin-α at the major NLS-binding site, indicating that the region of importin-α outside the NLS-binding site, possibly the autoinhibotory importin-β1-binding domain, may be the key determinant for the preferential binding of RanBP3 to importin-α3. Computational docking simulation indicates that phosphorylation of RanBP3 at Ser58 could potentially stabilize the association of RanBP3 with importin-α through interactions between the phosphate moiety of phospho-Ser58 of RanBP3 and a cluster of basic residues (Arg96 and Lys97 in importin-α3) on armadillo repeat 1 of importin-α.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_identifier_60":{"attribute_name":"URI","attribute_value_mlt":[{"subitem_identifier_type":"DOI","subitem_identifier_uri":"http://doi.org/10.1016/j.bbrc.2017.07.155"},{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/2237/27172"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2017.07.155","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Koyama, Masako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74381","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuura, Yoshiyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"74382","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-09-23"}],"displaytype":"detail","filename":"koyama2017BBRC_for_depository.pdf","filesize":[{"value":"2.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"koyama2017BBRC_for_depository.pdf ファイル公開日:2018/09/23","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/24950/files/koyama2017BBRC_for_depository.pdf"},"version_id":"7d154348-b328-44dc-a434-8e357429bb79"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"nuclear import","subitem_subject_scheme":"Other"},{"subitem_subject":"importin","subitem_subject_scheme":"Other"},{"subitem_subject":"NLS","subitem_subject_scheme":"Other"},{"subitem_subject":"RanBP3","subitem_subject_scheme":"Other"},{"subitem_subject":"phosphorylation","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["696"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2017-12-19"},"publish_date":"2017-12-19","publish_status":"0","recid":"24950","relation_version_is_last":true,"title":["Crystal structure of importin-a3 bound to the nuclear localization signal of Ran-binding protein 3"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:15:37.172121+00:00"}