{"_buckets": {"deposit": "8625137d-1f0f-4aad-95bd-32530fa1f946"}, "_deposit": {"id": "25022", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25022"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025022"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-10-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "1548.e3", "bibliographicPageStart": "1540", "bibliographicVolumeNumber": "25", "bibliographic_titles": [{"bibliographic_title": "STRUCTURE"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The flagellar motor protein complex consists of rotor and stator proteins. Their interaction generates torque of flagellum, which rotates bidirectionally, clockwise (CW) and counterclockwise. FliG, one of the rotor proteins, consists of three domains: N-terminal (FliGN), middle (FliGM), and C-terminal (FliGC). We have identified point mutations in FliGC from Vibrio alginolyticus, which affect the flagellar motility. To understand the molecular mechanisms, we explored the structural and dynamic properties of FliGC from both wild-type and motility-defective mutants. From nuclear magnetic resonance analysis, changes in signal intensities and chemical shifts between wild-type and the CW-biased mutant FliGC are observed in the C\u03b11-6 domain. Molecular dynamics simulations indicated the conformational dynamics of FliGC at sub-microsecond timescale, but not in the CW-biased mutant. Accordingly, we infer that the dynamic properties of atomic interactions around helix \u03b11 in the C\u03b11-6 domain of FliGC contribute to ensure the precise regulation of the motor switching.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1016/j.str.2017.08.010"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27244"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Elsevier"}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u00a9 2017. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "0969-2126", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Miyanoiri, Yohei"}], "nameIdentifiers": [{"nameIdentifier": "74583", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hijikata, Atsushi"}], "nameIdentifiers": [{"nameIdentifier": "74584", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishino, Yuuki"}], "nameIdentifiers": [{"nameIdentifier": "74585", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Gohara, Mizuki"}], "nameIdentifiers": [{"nameIdentifier": "74586", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Onoue, Yasuhiro"}], "nameIdentifiers": [{"nameIdentifier": "74587", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kojima, Seiji"}], "nameIdentifiers": [{"nameIdentifier": "74588", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kojima, Chojiro"}], "nameIdentifiers": [{"nameIdentifier": "74589", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shirai, Tsuyoshi"}], "nameIdentifiers": [{"nameIdentifier": "74590", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kainosho, Masatsune"}], "nameIdentifiers": [{"nameIdentifier": "74591", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Homma, Michio"}], "nameIdentifiers": [{"nameIdentifier": "74592", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-10-03"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Miyanoiri-MS1-Structure-rev8.pdf", "filesize": [{"value": "5.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 5000000.0, "url": {"label": "Miyanoiri-MS1-Structure-rev8.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5:2018/10/03", "url": "https://nagoya.repo.nii.ac.jp/record/25022/files/Miyanoiri-MS1-Structure-rev8.pdf"}, "version_id": "5a08bb12-2569-42ab-871d-0adb24516e5a"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "FliG", "subitem_subject_scheme": "Other"}, {"subitem_subject": "flagellar motor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NMR", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rotor", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Structural and Functional Analysis of the C-Terminal Region of FliG, an Essential Motor Component of Vibrio Na+-Driven Flagella", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Structural and Functional Analysis of the C-Terminal Region of FliG, an Essential Motor Component of Vibrio Na+-Driven Flagella"}]}, "item_type_id": "10", "owner": "1", "path": ["336/695/696"], "permalink_uri": "http://hdl.handle.net/2237/27244", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2018-01-10"}, "publish_date": "2018-01-10", "publish_status": "0", "recid": "25022", "relation": {}, "relation_version_is_last": true, "title": ["Structural and Functional Analysis of the C-Terminal Region of FliG, an Essential Motor Component of Vibrio Na+-Driven Flagella"], "weko_shared_id": null}