{"_buckets": {"deposit": "c8bcd151-4be1-4ec1-8b2d-b14eac62eb4c"}, "_deposit": {"id": "25139", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "25139"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00025139"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2017-08-07", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "3338", "bibliographicPageStart": "3332", "bibliographicVolumeNumber": "7", "bibliographic_titles": [{"bibliographic_title": "Catalysis Science and Technology"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The hydroxylation of non-native substrates catalysed by wild-type P450BM3 is reported, wherein \u201cdecoy molecules\u201d, i.e., native substrate mimics, controlled the stereoselectivity of hydroxylation reactions. We employed decoy molecules with diverse structures, resulting in either a significant improvement in enantioselectivity or clear inversion of stereoselectivity in the benzylic hydroxylation of alkylbenzenes and cycloalkylbenzenes. For example, supplementation of wild-type P450BM3 with 5-cyclohexylvaleric acid-L-phenylalanine (5CHVA-Phe) and Z-proline-L-phenylalanine yielded 53% (R) ee and 56% (S) ee for indane hydroxylation, respectively, although 16% (S) ee was still observed in the absence of any additives. Moreover, we performed a successful crystal structure analysis of 5CHVA-L-tryptophan-bound P450BM3 at 2.00 \u00c5, which suggests that the changes in selectivity observed were caused by conformational changes in the enzyme induced by binding of the decoy molecules.", "subitem_description_type": "Abstract"}]}, "item_10_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "DOI", "subitem_identifier_uri": "http://doi.org/10.1039/C7CY01130J"}, {"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/27359"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Royal Society of Chemistry"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_7": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "2044-4753", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Suzuki, Kazuto"}], "nameIdentifiers": [{"nameIdentifier": "75025", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kyle, Stanfield  Joshua"}], "nameIdentifiers": [{"nameIdentifier": "75026", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shoji, Osami"}], "nameIdentifiers": [{"nameIdentifier": "75027", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yanagisawa, Sota"}], "nameIdentifiers": [{"nameIdentifier": "75028", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sugimoto, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "75029", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Shiro, Yoshitsugu"}], "nameIdentifiers": [{"nameIdentifier": "75030", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Watanabe, Yoshihito"}], "nameIdentifiers": [{"nameIdentifier": "75031", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-08-07"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "RSC_draft_final_Josh.pdf", "filesize": [{"value": "1.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1400000.0, "url": {"label": "RSC_draft_final_Josh.pdf \u30d5\u30a1\u30a4\u30eb\u516c\u958b\u65e5:2018/08/07", "url": "https://nagoya.repo.nii.ac.jp/record/25139/files/RSC_draft_final_Josh.pdf"}, "version_id": "aca3ed41-89f1-4321-bc55-0db8bc655889"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Control of stereoselectivity of benzylic hydroxylation catalysed by wild-type cytochrome P450BM3 using decoy molecules", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Control of stereoselectivity of benzylic hydroxylation catalysed by wild-type cytochrome P450BM3 using decoy molecules"}]}, "item_type_id": "10", "owner": "1", "path": ["336/695/696"], "permalink_uri": "http://hdl.handle.net/2237/27359", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2018-02-05"}, "publish_date": "2018-02-05", "publish_status": "0", "recid": "25139", "relation": {}, "relation_version_is_last": true, "title": ["Control of stereoselectivity of benzylic hydroxylation catalysed by wild-type cytochrome P450BM3 using decoy molecules"], "weko_shared_id": null}