{"created":"2021-03-01T06:33:13.257319+00:00","id":25215,"links":{},"metadata":{"_buckets":{"deposit":"d2e2cff4-16d5-479d-9395-58e8d736a0be"},"_deposit":{"id":"25215","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25215"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00025215","sets":["643:666:667"]},"author_link":["75272","75273","75274","75275"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2017-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"899","bibliographicPageStart":"885","bibliographicVolumeNumber":"22","bibliographic_titles":[{"bibliographic_title":"Genes to Cells","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Milk fat globule-EGF factor 8 (MFG-E8) is a divalent-binding secretory protein possessing an Arg-Gly-Asp (RGD) motif and a phosphatidylserine (PS)-binding motif. This protein has been shown to be involved in mammary gland development and morphogenesis. Integrin-binding activity is necessary for these MFG-E8-dependent cell processes. Although the target cells and molecules of MFG-E8 in the cellular microenvironment are important to understand its physiological function, its localization is largely unclear. Here, we found that mouse MFG-E8 localized to the basal lamina of the mammary gland during involution. In a model system of mammary COMMA-1D cells, exogenously and endogenously expressed MFG-E8 was deposited in the extracellular matrix (ECM) with membranous particles dependently on the PS-binding motifs in the discoidin domains that were essential for association ability to extracellular vesicles (EVs). These data showed the basal MFG-E8 localization mechanism in which EVs served as a scaffold. Such an immobilized MFG-E8 associating with cell substrata but not soluble one in the culture media promoted integrin-dependent suppression of β-casein expression. These results suggest that MFG-E8 requires EVs to transduce cellular signals from the basolateral side of the adhesion cells by accumulating in ECM.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開日: 2018/10/01","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1111/gtc.12521","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is the pre-peer reviewed version of the following article: [Ooishi, T., Nadano, D., Matsuda, T. and Oshima, K. (2017), Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells. Genes Cells, 22: 885–899. doi:10.1111/gtc.12521], which has been published in final form at [http://doi.org/10.1111/gtc.12521]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"1356-9597","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ooishi, Takuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"75272","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nadano, Daita","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"75273","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuda, Tsukasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"75274","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oshima, Kenzi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"75275","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-10-01"}],"displaytype":"detail","filename":"Ooishi_et_al_revision_deposit_1.pdf","filesize":[{"value":"282.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Ooishi_et_al_revision_deposit_1","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/25215/files/Ooishi_et_al_revision_deposit_1.pdf"},"version_id":"d7d1b85d-bf35-4640-83fe-5632407cc085"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2018-10-01"}],"displaytype":"detail","filename":"Figs_deposit.pdf","filesize":[{"value":"6.6 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Figs_deposit","objectType":"dataset","url":"https://nagoya.repo.nii.ac.jp/record/25215/files/Figs_deposit.pdf"},"version_id":"78aec5e5-e4e8-40d3-9b9c-dd4c74824491"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"MFG-E8","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular matrix","subitem_subject_scheme":"Other"},{"subitem_subject":"extracellular vesicle","subitem_subject_scheme":"Other"},{"subitem_subject":"integrin","subitem_subject_scheme":"Other"},{"subitem_subject":"mammary gland","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-03-15"},"publish_date":"2018-03-15","publish_status":"0","recid":"25215","relation_version_is_last":true,"title":["Extracellular vesicle-mediated MFG-E8 localization in the extracellular matrix is required for its integrin-dependent function in mouse mammary epithelial cells"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:54:34.546001+00:00"}