{"created":"2021-03-01T06:33:47.941076+00:00","id":25752,"links":{},"metadata":{"_buckets":{"deposit":"858c2552-ffa0-4cf8-a24b-04f57c524b08"},"_deposit":{"id":"25752","owners":[],"pid":{"revision_id":0,"type":"depid","value":"25752"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00025752","sets":["2081:2082:2083"]},"author_link":["76401","76402","76403","76404"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"283","bibliographicPageStart":"274","bibliographicVolumeNumber":"592","bibliographic_titles":[{"bibliographic_title":"FEBS Letters","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Voltage‐gated sodium channels are crucial for electro‐signalling in living systems. Analysis of the molecular mechanism requires both fine electrophysiological evaluation and high‐resolution channel structures. Here, we optimized a dual expression system of NavAb, which is a well‐established standard of prokaryotic voltage‐gated sodium channels, for E. coli and insect cells using a single plasmid vector to analyse high‐resolution protein structures and measure large ionic currents. Using this expression system, we evaluated the voltage dependence and determined the crystal structures of NavAb wild‐type and two mutants, E32Q and N49K, whose voltage dependence were positively shifted and essential interactions were lost in voltage sensor domain. The structural and functional comparison elucidated the molecular mechanisms of the voltage dependence of prokaryotic voltage‐gated sodium channels.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開：2019-01-09 ","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/1873-3468.12955","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is the peer reviewed version of the following article: [Irie, K. , Haga, Y. , Shimomura, T. and Fujiyoshi, Y. (2018), Optimized expression and purification of NavAb provide the structural insight into the voltage dependence. FEBS Lett, 592: 274-283. doi:10.1002/1873-3468.12955], which has been published in final form at [https://doi.org/10.1002/1873-3468.12955]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00145793","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Irie, Katsumasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"76401","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Haga, Yukari","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"76402","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimomura, Takushi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"76403","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Fujiyoshi, Yoshinori","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"76404","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-01-09"}],"displaytype":"detail","filename":"Revised_FEBSL-17-1362_R1_proof_kirie_docx.pdf","filesize":[{"value":"3.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Revised_FEBSL-17-1362_R1_proof_kirie_docx","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/25752/files/Revised_FEBSL-17-1362_R1_proof_kirie_docx.pdf"},"version_id":"e1b8576d-2017-4c85-97f1-b1a3c982ab58"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"protein expression","subitem_subject_scheme":"Other"},{"subitem_subject":"structural physiology","subitem_subject_scheme":"Other"},{"subitem_subject":"voltage‐gated sodium channel","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Optimized expression and purification of NavAb provide the structural insight into the voltage dependence","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Optimized expression and purification of NavAb provide the structural insight into the voltage dependence","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["2083"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-05-10"},"publish_date":"2018-05-10","publish_status":"0","recid":"25752","relation_version_is_last":true,"title":["Optimized expression and purification of NavAb provide the structural insight into the voltage dependence"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:30:24.942486+00:00"}