@article{oai:nagoya.repo.nii.ac.jp:00026086, author = {Uchida, Naoyuki and Takahashi, Koji and Iwasaki, Rie and Yamada, Ryotaro and Yoshimura, Masahiko and Endo, Takaho A and Kimura, Seisuke and Zhang, Hua and Nomoto, Mika and Tada, Yasuomi and Kinoshita, Toshinori and Itami, Kenichiro and Hagihara, Shinya and Torii, Keiko U}, issue = {3}, journal = {Nature Chemical Biology}, month = {Jan}, note = {The phytohormone auxin indole-3-acetic acid (IAA) regulates nearly all aspects of plant growth and development. Despite substantial progress in our understanding of auxin biology, delineating specific auxin response remains a major challenge. Auxin regulates transcriptional response via its receptors, TIR1 and AFB F-box proteins. Here we report an engineered, orthogonal auxin–TIR1 receptor pair, developed through a bump-and-hole strategy, that triggers auxin signaling without interfering with endogenous auxin or TIR1/AFBs. A synthetic, convex IAA (cvxIAA) hijacked the downstream auxin signaling in vivo both at the transcriptomic level and in specific developmental contexts, only in the presence of a complementary, concave TIR1 (ccvTIR1) receptor. Harnessing the cvxIAA–ccvTIR1 system, we provide conclusive evidence for the role of the TIR1-mediated pathway in auxin-induced seedling acid growth. The cvxIAA–ccvTIR1 system serves as a powerful tool for solving outstanding questions in auxin biology and for precise manipulation of auxin-mediated processes as a controllable switch., ファイル公開:2018-07-22}, pages = {299--305}, title = {Chemical hijacking of auxin signaling with an engineered auxin–TIR1 pair}, volume = {14}, year = {2018} }