@article{oai:nagoya.repo.nii.ac.jp:00026107, author = {Liu, Zeyang and Lu, Minmin and Takeuchi, Masaru and Yue, Tao and Hasegawa, Yasuhisa and Huang, Qiang and Fukuda, Toshio}, issue = {3}, journal = {Biomedical Materials}, month = {May}, note = {Artificial cell sheets are commonly utilized as buiding blocks for tissue engineering. We propose a novel approach in the fabrication of Ca-alginate gel sheets, embedded with liver cells (RLC-18) in order to mimic liver lobule tissue. Ca-alginate sheets with hepatic lobule-shaped patterns were deposited onto a micro-electrode device using electrodeposition. Viability of embedded cells was ensured to exceed 80%. Cell morphology and biofunctionality were monitored during the one-week culture period and results compared with those of traditional 2D culture. In addition, we detached cell sheets from the electrode substrate and stacked them into a 3D multi-layered structure to mimic the morphology of liver lobule tissue., ファイル公開:2019-05-01}, title = {In vitro mimicking the morphology of hepatic lobule tissue based on Ca-alginate cell sheets}, volume = {13}, year = {2018} }