{"created":"2021-03-01T06:34:01.095253+00:00","id":26123,"links":{},"metadata":{"_buckets":{"deposit":"77f17107-2854-4325-b1f4-eff52277d2fc"},"_deposit":{"id":"26123","owners":[],"pid":{"revision_id":0,"type":"depid","value":"26123"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00026123","sets":["336:695:696"]},"author_link":["78364","78365","78366","78367","78368"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-02-06","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"497","bibliographicPageStart":"494","bibliographicVolumeNumber":"57","bibliographic_titles":[{"bibliographic_title":"Biochemistry","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The light oxygen voltage-sensing (LOV) domain plays a crucial role in blue light (BL) sensing in plants and microorganisms. LOV domains are usually associated with the effector domains and regulate the activities of effector domains in a BL-dependent manner. Photozipper (PZ) is monomeric in the dark state. BL induces reversible dimerization of PZ and subsequently increases its affinity for the target DNA sequence. In this study, we report the analyses of PZ by pulsed electron–electron double resonance (PELDOR). The neutral flavin radical was formed by BL illumination in the presence of dithiothreitol in the LOV-C254S (without the bZIP domain) and PZ-C254S mutants, where the cysteine residue responsible for adduct formation was replaced with serine. The magnetic dipole interactions of 3 MHz between the neutral radicals were detected in both LOV-C254S and PZ-C254S, indicating that these mutants are dimeric in the radical state. The PELDOR simulation showed that the distance between the radical pair is close to that estimated from the dimeric crystal structure in the “light state” [Heintz, U., and Schlichting, I. (2016) eLife 5, e11860], suggesting that in the radical state, LOV domains in PZ-C254S form a dimer similar to that of LOV-C254S, which lacks the bZIP domain.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開：2019/02/06","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS Publications","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1021/acs.biochem.7b01045","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Biochemistry], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [http://pubs.acs.org/articlesonrequest/AOR-3F5DaaWT3x2Ir9KDijgv].” ","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0006-2960","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ozeki, Kohei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"78364","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tsukuno, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"78365","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nagashima, Hiroki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"78366","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hisatomi, Osamu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"78367","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mino, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"78368","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-02-06"}],"displaytype":"detail","filename":"manuscriptPELDORforRep.pdf","filesize":[{"value":"410.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"manuscriptPELDORforRep","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/26123/files/manuscriptPELDORforRep.pdf"},"version_id":"70715470-88aa-4c80-b6a0-93ef7642de68"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["696"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-07-02"},"publish_date":"2018-07-02","publish_status":"0","recid":"26123","relation_version_is_last":true,"title":["Dimeric Structure of the Blue Light Sensor Protein Photozipper in the Active State"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:16:38.079976+00:00"}