{"created":"2021-03-01T06:34:15.152507+00:00","id":26335,"links":{},"metadata":{"_buckets":{"deposit":"9188ac9f-3cbd-439c-97e0-2aa395ca8f07"},"_deposit":{"id":"26335","owners":[],"pid":{"revision_id":0,"type":"depid","value":"26335"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00026335","sets":["643:666:667"]},"author_link":["85106","85107","85108","85109","85110","85111"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"322","bibliographicPageStart":"312","bibliographicVolumeNumber":"36","bibliographic_titles":[{"bibliographic_title":"Cell Biochemistry and Function","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"While transcription plays an obviously important role in gene expression, translation has recently been emerged as a key step that defines the composition and quality of the proteome in the cell of higher eukaryotes including mammals. Selective translation is supposed to be regulated by the structural heterogeneity of cytoplasmic ribosomes including differences in protein composition and chemical modifications. However, the current knowledge on the heterogeneity of mammalian ribosomes is limited. Here, we report mammalian Serbp1 as a ribosome‐associated protein. The translated products of Serbp1 gene, including the longest isoform, were found to be localized in the nucleolus as well as in the cytoplasm. Subcellular fractionation indicated that most of cytoplasmic Serbp1 molecules were precipitated by ultracentrifugation. Proteomic analysis identified Serbp1 in the cytoplasmic ribosomes of the rodent testis. Polysome profiling suggested that Serbp1, as a component of the small 40S subunit, was included in translating ribosomes (polysomes). Cosedimentation of Serbp1 with the 40S subunit was observed after dissociation of the ribosomal subunits. Serbp1 was also included in the ribosomes of human cancer cells, which may lead to a mechanistic understanding of an emerging link between Serbp1 and tumour progression.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開：2019/08/01","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Wiley","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1002/cbf.3350","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This is the peer reviewed version of the following article: [Muto A, Sugihara Y, Shibakawa M, Oshima K, Matsuda T, Nadano D. The mRNA‐binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes. Cell Biochem Funct. 2018;36:312–322. https://doi.org/10.1002/cbf.3350], which has been published in final form at [https://doi.org/10.1002/cbf.3350]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. ","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_7":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"0263-6484","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Muto, Akiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85106","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sugihara, Yoshihiko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85107","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shibakawa, Minami","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85108","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oshima, Kenzi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85109","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Matsuda, Tsukasa","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85110","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nadano, Daita","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85111","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-01"}],"displaytype":"detail","filename":"Serbp1-201808-op.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Serbp1-201808-op","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/26335/files/Serbp1-201808-op.pdf"},"version_id":"98af9ce7-f0ce-4a6c-a0be-e0ee3962730b"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"cytoplasmic ribosome","subitem_subject_scheme":"Other"},{"subitem_subject":"gene expression","subitem_subject_scheme":"Other"},{"subitem_subject":"isoform","subitem_subject_scheme":"Other"},{"subitem_subject":"malignant tumour","subitem_subject_scheme":"Other"},{"subitem_subject":"mammal","subitem_subject_scheme":"Other"},{"subitem_subject":"mRNA‐binding protein","subitem_subject_scheme":"Other"},{"subitem_subject":"polysome","subitem_subject_scheme":"Other"},{"subitem_subject":"translational control","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"The mRNA-binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"The mRNA-binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-09-05"},"publish_date":"2018-09-05","publish_status":"0","recid":"26335","relation_version_is_last":true,"title":["The mRNA-binding protein Serbp1 as an auxiliary protein associated with mammalian cytoplasmic ribosomes"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:54:44.374611+00:00"}