{"created":"2021-03-01T06:34:18.241603+00:00","id":26379,"links":{},"metadata":{"_buckets":{"deposit":"0e8fa7a9-96a2-4096-958b-46ee0311f3c2"},"_deposit":{"id":"26379","owners":[],"pid":{"revision_id":0,"type":"depid","value":"26379"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00026379","sets":["643:666:667"]},"author_link":["85359","85360","85361","85362","85363","85364","85365","85366","85367","85368"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-05-18","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5","bibliographicPageEnd":"1269","bibliographicPageStart":"1260","bibliographicVolumeNumber":"13","bibliographic_titles":[{"bibliographic_title":"ACS Chemical Biology","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"From the RIKEN Natural Products Depository (NPDepo) chemical library, we identified small molecules that alter trichothecene 15-acetyldeoxynivalenol (15-ADON) production by Fusarium graminearum. Among trichothecene production activators, a furanocoumarin NPD12671 showed the strongest stimulatory activity on 15-ADON production by the fungus cultured in a 24-well plate. NPD12671 significantly increased the transcription of Tri6, a transcription factor gene necessary for trichothecene biosynthesis, in both trichothecene-inducing and noninducing culture conditions. Dihydroartemisinin (DHA) was identified as the most effective inhibitor of trichothecene production in 24-well plate culture; DHA inhibited trichothecene production (>50% inhibition at 1 μM) without affecting fungal mass by suppressing Tri6 expression. To determine the effect of DHA on trichothecene pathway Tri gene expression, we generated a constitutively Tri6-overexpressing strain that produced 15-ADON in YG_60 medium in Erlenmeyer flasks, conditions under which no trichothecenes are produced by the wild-type. While 5 μM DHA failed to inhibit trichothecene biosynthesis by the overexpressor in trichothecene-inducing YS_60 culture, trichothecene production was suppressed in the YG_60 culture. Regardless of a high Tri6 transcript level in the constitutive overexpressor, the YG_60 culture showed reduced accumulation of Tri5 and Tri4 mRNA upon treatment with 5 μM DHA. Deletion mutants of FgOs2 were also generated and examined; both NPD12671 and DHA modulated trichothecene production as they did in the wild-type strain. These results are discussed in light of the mode of actions of these chemicals on trichothecene biosynthesis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開：2019-05-18 ","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS Publications","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1021/acschembio.8b00044","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Chemical Biology, copyright© American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/articlesonrequest/AOR-eg9SCN4f38Gp55nxRPq7","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_61":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"1554-8929","subitem_source_identifier_type":"PISSN"}]},"item_10_source_id_62":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"1554-8937","subitem_source_identifier_type":"EISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Maeda, Kazuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85359","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ichikawa, Hinayo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85360","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakajima, Yuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85361","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Motoyama, Takayuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85362","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohsato, Shuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85363","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanamaru, Kyoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85364","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Tetsuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85365","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishiuchi, Takumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85366","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Osada, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85367","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimura, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"85368","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-05-18"}],"displaytype":"detail","filename":"Identification_and_characterization_of_small_molecule_compounds.pdf","filesize":[{"value":"3.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Identification_and_characterization_of_small_molecule_compounds","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/26379/files/Identification_and_characterization_of_small_molecule_compounds.pdf"},"version_id":"5c4fc8f1-f5e9-413c-a112-3d130bbd5c7b"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-09-27"},"publish_date":"2018-09-27","publish_status":"0","recid":"26379","relation_version_is_last":true,"title":["Identification and Characterization of Small Molecule Compounds That Modulate Trichothecene Production by Fusarium graminearum"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:16:55.833509+00:00"}