{"created":"2021-03-01T06:34:19.372706+00:00","id":26396,"links":{},"metadata":{"_buckets":{"deposit":"071d19d5-7f0a-402c-be3a-de10e7b43a4c"},"_deposit":{"id":"26396","owners":[],"pid":{"revision_id":0,"type":"depid","value":"26396"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00026396","sets":["336:695:696"]},"author_link":["86727","86728","86729","86730","86731"],"control_number":"26396","item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2018-09-27","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"38","bibliographicPageEnd":"8823","bibliographicPageStart":"8819","bibliographicVolumeNumber":"122","bibliographic_titles":[{"bibliographic_title":"The Journal of Physical Chemistry B","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Formation of the neutral flavin radical in the light-oxygen-voltage-sensing (LOV-sensing) domain of photozipper, based on VfAUREO1, was investigated by electron paramagnetic resonance spectroscopy. The flavin radical was observed in the presence of dithiothreitol by illumination of a LOV-domain mutant (C254S), in which a photoactive cysteine residue in close proximity to flavin was replaced with a serine. The radical did not form under low initial protein-concentration conditions (less than 20 μM). The flavin radicals accumulated with logistic time-dependent kinetics when the protein concentrations were higher than 30 μM. These results indicate that the radical is produced by concerted reactions involving protein interactions and that the radical is formed from the LOV dimer but not the LOV monomer. In contrast, logistic time dependencies were not observed for the sample adapted to the dark following radical formation by illumination, indicating that initialization of the proton pathway is essential for this fast sensing reaction.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開：2019-09-27","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS Publications","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1021/acs.jpcb.8b05808","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [The Journal of Physical Chemistry B], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-Qq5gDsJQwhhUHFdDV7Mv].”","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_61":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"1520-6106","subitem_source_identifier_type":"PISSN"}]},"item_10_source_id_62":{"attribute_name":"ISSN（Online）","attribute_value_mlt":[{"subitem_source_identifier":"1520-5207","subitem_source_identifier_type":"EISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Tsukuno, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"86727","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ozeki, Kohei","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"86728","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Itsuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"86729","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hisatomi, Osamu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"86730","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mino, Hiroyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"86731","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-27"}],"displaytype":"detail","filename":"180826_photozippermanusctipt.pdf","filesize":[{"value":"435.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"180826_photozippermanusctipt","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/26396/files/180826_photozippermanusctipt.pdf"},"version_id":"4418668e-be03-4530-840f-2ee35727fefc"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-09-27"}],"displaytype":"detail","filename":"jp8b05808_si_001.pdf","filesize":[{"value":"876.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"jp8b05808_si_001","objectType":"dataset","url":"https://nagoya.repo.nii.ac.jp/record/26396/files/jp8b05808_si_001.pdf"},"version_id":"66a9ff2a-bfd1-42d3-8800-0cde1bbd178f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Flavin-Radical Formation in the Light-Oxygen-Voltage-Sensing Domain of the Photozipper Blue-light Sensor Protein","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Flavin-Radical Formation in the Light-Oxygen-Voltage-Sensing Domain of the Photozipper Blue-light Sensor Protein","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["696"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2018-10-09"},"publish_date":"2018-10-09","publish_status":"0","recid":"26396","relation_version_is_last":true,"title":["Flavin-Radical Formation in the Light-Oxygen-Voltage-Sensing Domain of the Photozipper Blue-light Sensor Protein"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:16:55.202344+00:00"}