@article{oai:nagoya.repo.nii.ac.jp:00028213, author = {Ando, Eigo and Kinoshita, Toshinori}, issue = {2}, journal = {Plant Signaling & Behavior}, month = {}, note = {Stomatal opening is induced by red light as well as blue light. Recently, we established an immunohistochemical technique using whole leaves to study plasma membrane (PM) H^+-ATPase in guard cells, which is an important enzyme driving stomatal opening. Our technique revealed that red light illuminated to whole leaves induces photosynthesis-dependent phosphorylation of C-terminal penultimate residue of PM H^+-ATPase, threonine, in guard cells, which has been considered to be important for activation of PM H^+-ATPase, and we proposed that red light promotes stomatal opening via activation of PM H^+-ATPase in guard cells in whole leaves. Here, using our new immunohistochemical technique, we investigated fluence rate dependence of red light-induced phosphorylation of PM H^+-ATPase. We found that illumination of red light at 50 µmol m^–2 s^–1, which was suggested to initiate photosynthesis, saturates phosphorylation of PM H^+-ATPase. Furthermore, we immunohistochemically confirmed decrease in the amount of PM H^+-ATPase protein in a knock-out mutant of AHA1, an isogene encoding the major isoform of PM H^+-ATPase in guard cells, implying the importance of AHA1 as the major PM H^+-ATPase protein in guard cells for light-induced stomatal opening., Published online: 02 Jan 2019. ファイル公開:2020/01/02}, title = {Fluence rate dependence of red light-induced phosphorylation of plasma membrane H^+-ATPase in stomatal guard cells}, volume = {14}, year = {2019} }