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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Inhibition of Fusarium trichothecene biosynthesis by yeast extract components extractable with ethyl acetate

http://hdl.handle.net/2237/00032177
http://hdl.handle.net/2237/00032177
bb88fa31-413a-45c2-bcc2-41e803b2256e
名前 / ファイル ライセンス アクション
Inhibition_of_Fusarium_trichothecene_biosynthesis.pdf Inhibition_of_Fusarium_trichothecene_biosynthesis (1.7 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-05-19
タイトル
タイトル Inhibition of Fusarium trichothecene biosynthesis by yeast extract components extractable with ethyl acetate
言語 en
著者 Tanaka, Yuya

× Tanaka, Yuya

WEKO 98991

en Tanaka, Yuya

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Nakajima, Yuichi

× Nakajima, Yuichi

WEKO 98992

en Nakajima, Yuichi

Search repository
Maeda, Kazuyuki

× Maeda, Kazuyuki

WEKO 98993

en Maeda, Kazuyuki

Search repository
Matsuyama, Momoko

× Matsuyama, Momoko

WEKO 98994

en Matsuyama, Momoko

Search repository
Kanamaru, Kyoko

× Kanamaru, Kyoko

WEKO 98995

en Kanamaru, Kyoko

Search repository
Kobayashi, Tetsuo

× Kobayashi, Tetsuo

WEKO 98996

en Kobayashi, Tetsuo

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Ohsato, Shuichi

× Ohsato, Shuichi

WEKO 98997

en Ohsato, Shuichi

Search repository
Kimura, Makoto

× Kimura, Makoto

WEKO 98998

en Kimura, Makoto

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
キーワード
主題Scheme Other
主題 Complex medium
キーワード
主題Scheme Other
主題 Fusarium graminearum
キーワード
主題Scheme Other
主題 Inhibitor
キーワード
主題Scheme Other
主題 Maillard reaction
キーワード
主題Scheme Other
主題 Trichothecene mycotoxin
キーワード
主題Scheme Other
主題 Yeast extract
抄録
内容記述 While Fusarium graminearum readily produces trichothecenes in complex media containing sucrose as the carbon source (YS_60), the amount of the mycotoxin is quite limited when other sugars, such as glucose and fructose, are used. We found that autoclaving of media containing fructose and yeast extract (YF_60) results in the formation of inhibitors of trichothecene biosynthesis by F. graminearum JCM 9873, a strain that produces 15-acetyldeoxynivalenol (15-ADON) in liquid culture. Removal of the solvent fraction from the autoclaved media after ethyl acetate extraction attenuated the inhibitory activity against trichothecene production. In addition, extraction of the non-autoclaved complex media with ethyl acetate, followed by removal of the solvent fraction, similarly resulted in increased accumulation of the mycotoxin. Although the increase in trichothecene production differed considerably among fungal strains and yeast extract products, F. graminearum species complex generally responded to the medium treatments in the same way. These results suggest that some hydrophobic substances that arise during the drying and heating of yeast extract negatively affected trichothecene production in liquid culture. Modes of actions of inhibitory substances were partially characterized using strain JCM 9873, with focus on the transcriptional and functional analyses of Tri6, a key regulator gene in trichothecene biosynthesis. The presence of the ethyl acetate-extractable substances in autoclaved YF_60 media decreased the relative transcription level of Tri6, as well as that of a trichodiene synthase gene Tri5. Thus, the substances exerted their inhibitory action through suppression of Tri6 expression. By using a yeast extract lot that completely prevented trichothecene production by the wild-type strain in autoclaved YS_60 medium, we prepared YF_60 media and cultured a constitutive Tri6 overexpressor strain described by Maeda et al. (2018). Despite the high transcription level of Tri6, the presence of the ethyl acetate extractable-substances suppressed 15-ADON production. These results suggested that both Tri6p-independent initial activation of Tri6 expression and subsequent Tri6p-dependent activation of Tri expression were affected by the hydrophobic substances in the yeast extract products.
言語 en
内容記述タイプ Abstract
出版者
言語 en
出版者 Elsevier
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1016/j.ijfoodmicro.2018.08.026
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 0168-1605
書誌情報 en : International Journal of Food Microbiology

巻 289, p. 24-29, 発行日 2019-01-16
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