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Inhibition of Fusarium trichothecene biosynthesis by yeast extract components extractable with ethyl acetate
http://hdl.handle.net/2237/00032177
http://hdl.handle.net/2237/00032177bb88fa31-413a-45c2-bcc2-41e803b2256e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
Inhibition_of_Fusarium_trichothecene_biosynthesis (1.7 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2020-05-19 | |||||
| タイトル | ||||||
| タイトル | Inhibition of Fusarium trichothecene biosynthesis by yeast extract components extractable with ethyl acetate | |||||
| 言語 | en | |||||
| 著者 |
Tanaka, Yuya
× Tanaka, Yuya× Nakajima, Yuichi× Maeda, Kazuyuki× Matsuyama, Momoko× Kanamaru, Kyoko× Kobayashi, Tetsuo× Ohsato, Shuichi× Kimura, Makoto |
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| アクセス権 | ||||||
| アクセス権 | open access | |||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
| 権利 | ||||||
| 言語 | en | |||||
| 権利情報 | © 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Complex medium | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Fusarium graminearum | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Inhibitor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Maillard reaction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Trichothecene mycotoxin | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Yeast extract | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | While Fusarium graminearum readily produces trichothecenes in complex media containing sucrose as the carbon source (YS_60), the amount of the mycotoxin is quite limited when other sugars, such as glucose and fructose, are used. We found that autoclaving of media containing fructose and yeast extract (YF_60) results in the formation of inhibitors of trichothecene biosynthesis by F. graminearum JCM 9873, a strain that produces 15-acetyldeoxynivalenol (15-ADON) in liquid culture. Removal of the solvent fraction from the autoclaved media after ethyl acetate extraction attenuated the inhibitory activity against trichothecene production. In addition, extraction of the non-autoclaved complex media with ethyl acetate, followed by removal of the solvent fraction, similarly resulted in increased accumulation of the mycotoxin. Although the increase in trichothecene production differed considerably among fungal strains and yeast extract products, F. graminearum species complex generally responded to the medium treatments in the same way. These results suggest that some hydrophobic substances that arise during the drying and heating of yeast extract negatively affected trichothecene production in liquid culture. Modes of actions of inhibitory substances were partially characterized using strain JCM 9873, with focus on the transcriptional and functional analyses of Tri6, a key regulator gene in trichothecene biosynthesis. The presence of the ethyl acetate-extractable substances in autoclaved YF_60 media decreased the relative transcription level of Tri6, as well as that of a trichodiene synthase gene Tri5. Thus, the substances exerted their inhibitory action through suppression of Tri6 expression. By using a yeast extract lot that completely prevented trichothecene production by the wild-type strain in autoclaved YS_60 medium, we prepared YF_60 media and cultured a constitutive Tri6 overexpressor strain described by Maeda et al. (2018). Despite the high transcription level of Tri6, the presence of the ethyl acetate extractable-substances suppressed 15-ADON production. These results suggested that both Tri6p-independent initial activation of Tri6 expression and subsequent Tri6p-dependent activation of Tri expression were affected by the hydrophobic substances in the yeast extract products. | |||||
| 言語 | en | |||||
| 出版者 | ||||||
| 出版者 | Elsevier | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 出版タイプ | ||||||
| 出版タイプ | AM | |||||
| 出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
| DOI | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | https://doi.org/10.1016/j.ijfoodmicro.2018.08.026 | |||||
| ISSN（print） | ||||||
| 収録物識別子タイプ | PISSN | |||||
| 収録物識別子 | 0168-1605 | |||||
| 書誌情報 |
en : International Journal of Food Microbiology 巻 289, p. 24-29, 発行日 2019-01-16 |
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| 著者版フラグ | ||||||
| 値 | author | |||||
