{"created":"2021-03-01T06:38:15.042508+00:00","id":29992,"links":{},"metadata":{"_buckets":{"deposit":"06d09c92-91d8-4120-8811-71be2927bddd"},"_deposit":{"id":"29992","owners":[],"pid":{"revision_id":0,"type":"depid","value":"29992"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00029992","sets":["643:666:667"]},"author_link":["98999","99000","99001","99002","99003","99004","99005","99006","99007","99008"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-03-15","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"3","bibliographicPageEnd":"478","bibliographicPageStart":"471","bibliographicVolumeNumber":"112","bibliographic_titles":[{"bibliographic_title":"Antonie van Leeuwenhoek","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Nitrogen sources in media have a significant impact on the onset of secondary metabolism in fungi. For transcriptional activation of many nitrogen catabolic genes, an AreA transcription factor is indispensable. This also holds true for Fusarium graminearum that produces trichothecenes, an important group of mycotoxin, in axenic culture. Despite the presence of numerous consensus AreA-binding sites in the promoters of Tri genes in the trichothecene cluster core region, the effect of medium amino acids on trichothecene biosynthesis is poorly understood. In this study, we examined the effect of certain amino acids, which were predicted to activate AreA function and increase Tri gene transcription, on trichothecene production in liquid culture. By frequent monitoring and adjustments in the pH of the culture medium, including replacement of the spent medium with fresh medium, we demonstrate the suppressive effects of the amino acids, used as the sole nitrogen source, on trichothecene biosynthesis. When the medium pH was maintained at 4.0, Gly, l-Ser, and l-Thr suppressed trichothecene production by F. graminearum. Enhanced trichothecene-inducing effects were observed when the medium pH was 3.5, with only l-Thr suppressing trichothecene synthesis.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Springer","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1007/s10482-018-1172-z","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"“This is a post-peer-review, pre-copyedit version of an article published in [Antonie van Leeuwenhoek]. The final authenticated version is available online at: http://dx.doi.org/10.1007/s10482-018-1172-z”.","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_61":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0003-6072","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Shiobara, Takuya","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"98999","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakajima, Yuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99000","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Maeda, Kazuyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99001","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Akasaka, Manami","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99002","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kitou, Yoshiyuki","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99003","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kanamaru, Kyoko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99004","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohsato, Shuichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99005","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kobayashi, Tetsuo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99006","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nishiuchi, Takumi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99007","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kimura, Makoto","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99008","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-05-19"}],"displaytype":"detail","filename":"Identification_of_amino_acids.pdf","filesize":[{"value":"3.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Identification_of_amino_acids","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/29992/files/Identification_of_amino_acids.pdf"},"version_id":"9ba8173b-b79a-4b5f-ad77-9c2e828d2109"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"AreA transcription factor","subitem_subject_scheme":"Other"},{"subitem_subject":"Defined medium","subitem_subject_scheme":"Other"},{"subitem_subject":"Fed-batch culture","subitem_subject_scheme":"Other"},{"subitem_subject":"Mycotoxin biosynthesis","subitem_subject_scheme":"Other"},{"subitem_subject":"Secondary metabolite","subitem_subject_scheme":"Other"},{"subitem_subject":"Threonine metabolism","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Identification of amino acids negatively affecting Fusarium trichothecene biosynthesis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Identification of amino acids negatively affecting Fusarium trichothecene biosynthesis","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-05-19"},"publish_date":"2020-05-19","publish_status":"0","recid":"29992","relation_version_is_last":true,"title":["Identification of amino acids negatively affecting Fusarium trichothecene biosynthesis"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:23:09.934818+00:00"}