{"created":"2021-03-01T06:38:19.812403+00:00","id":30061,"links":{},"metadata":{"_buckets":{"deposit":"d7320536-d586-441d-9133-e144c9fc6f50"},"_deposit":{"id":"30061","owners":[],"pid":{"revision_id":0,"type":"depid","value":"30061"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00030061","sets":["643:666:667"]},"author_link":["99129","99130","99131","99132"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-12-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"296","bibliographicPageStart":"291","bibliographicVolumeNumber":"520","bibliographic_titles":[{"bibliographic_title":"Biochemical and Biophysical Research Communications","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Cis-prenyltransferases are enzymes responsible for the biosynthesis of glycosyl carrier lipids, natural rubber, and some secondary metabolites. Certain organisms, including some archaeal species, possess multiple genes encoding cis-prenyltransferase homologs, and the physiological roles of these seemingly-redundant genes are often obscure. Cis-prenyltransferases usually form homomeric complexes, but recent reports have demonstrated that certain eukaryotic enzymes are heteromeric protein complexes consisting of two homologous subunits. In this study, three cis-prenyltransferase homolog proteins, MM_0014, MM_0618, and MM_1083, from the methanogenic archaeon Methanosarcina mazei are overexpressed in Escherichia coli and partially purified for functional characterization. Coexistence of MM_0618 and MM_1083 exhibits prenyltransferase activity, while each of them alone has almost no activity. The chain-lengths of the products of this heteromeric enzyme are in good agreement with those of glycosyl carrier lipids extracted from M. mazei, which are likely di- and tetra-hydrogenated decaprenyl phosphates, suggesting that the MM_0618/MM_1083 heteromer is involved in glycosyl carrier lipid biosynthesis. MM_0014 acts as a typical homomeric cis-prenyltransferase and produces shorter products.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開：2020-12-03","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.bbrc.2019.09.143","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2019. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_61":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0006-291X","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Emi, Koh-ichi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99129","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sompiyachoke, Kitty","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99130","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Okada, Miyako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99131","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hemmi, Hisashi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"99132","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-12-03"}],"displaytype":"detail","filename":"for_institutional_repository.pdf","filesize":[{"value":"1.8 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"for_institutional_repository","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/30061/files/for_institutional_repository.pdf"},"version_id":"a19d5ae9-5f4a-400d-a091-ad53446e6f19"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Cis-prenyltransferase","subitem_subject_scheme":"Other"},{"subitem_subject":"Archaea","subitem_subject_scheme":"Other"},{"subitem_subject":"Glycosyl carrier lipid","subitem_subject_scheme":"Other"},{"subitem_subject":"Heteromer","subitem_subject_scheme":"Other"},{"subitem_subject":"Isoprenoid","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"A heteromeric cis-prenyltransferase is responsible for the biosynthesis of glycosyl carrier lipids in Methanosarcina mazei","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"A heteromeric cis-prenyltransferase is responsible for the biosynthesis of glycosyl carrier lipids in Methanosarcina mazei","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["667"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-05-25"},"publish_date":"2020-05-25","publish_status":"0","recid":"30061","relation_version_is_last":true,"title":["A heteromeric cis-prenyltransferase is responsible for the biosynthesis of glycosyl carrier lipids in Methanosarcina mazei"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:23:17.043354+00:00"}