{"created":"2021-03-01T06:38:38.686153+00:00","id":30336,"links":{},"metadata":{"_buckets":{"deposit":"a02af64f-d298-44cd-9d56-c8798e8cc56d"},"_deposit":{"id":"30336","owners":[],"pid":{"revision_id":0,"type":"depid","value":"30336"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00030336","sets":["329:330:331"]},"author_link":["100646","100647","100648","100649","100650","100651","100652","100653","100654","100655","100656","100657","100658","100659","100660","100661","100662","100663","100664","100665","100666","100667","100668","100669","100670","100671"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-03-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"6","bibliographicPageEnd":"1244.e24","bibliographicPageStart":"1228","bibliographicVolumeNumber":"180","bibliographic_titles":[{"bibliographic_title":"Cell","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Transcription-coupled nucleotide excision repair (TC-NER) is initiated by the stalling of elongating RNA polymerase II (RNAPIIo) at DNA lesions. The ubiquitination of RNAPIIo in response to DNA damage is an evolutionarily conserved event, but its function in mammals is unknown. Here, we identified a single DNA damage-induced ubiquitination site in RNAPII at RPB1-K1268, which regulates transcription recovery and DNA damage resistance. Mechanistically, RPB1-K1268 ubiquitination stimulates the association of the core-TFIIH complex with stalled RNAPIIo through a transfer mechanism that also involves UVSSA-K414 ubiquitination. We developed a strand-specific ChIP-seq method, which revealed RPB1-K1268 ubiquitination is important for repair and the resolution of transcriptional bottlenecks at DNA lesions. Finally, RPB1-K1268R knockin mice displayed a short life-span, premature aging, and neurodegeneration. Our results reveal RNAPII ubiquitination provides a two-tier protection mechanism by activating TC-NER and, in parallel, the processing of DNA damage-stalled RNAPIIo, which together prevent prolonged transcription arrest and protect against neurodegeneration.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開：2021-03-19","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.cell.2020.02.010","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2020. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_61":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"0092-8674","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Nakazawa, Yuka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100646","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hara, Yuichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100647","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Oka, Yasuyoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100648","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Komine, Okiru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100649","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"van den Heuvel, Diana","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100650","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Guo, Chaowan","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100651","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Daigaku, Yasukazu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100652","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Isono, Mayu","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100653","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"He, Yuxi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100654","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Shimada, Mayuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100655","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kato, Kana","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100656","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Jia, Nan","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100657","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Hashimoto, Satoru","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100658","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Kotani, Yuko","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100659","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Miyoshi, Yuka","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100660","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tanaka, Miyako","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100661","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Sobue, Akira","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100662","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mitsutake, Norisato","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100663","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Suganami, Takayoshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100664","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Masuda, Akio","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100665","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ohno, Kinji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100666","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Nakada, Shinichiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100667","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Mashimo, Tomoji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100668","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Yamanaka, Koji","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100669","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Luijsterburg, Martijn S.","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100670","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Ogi, Tomoo","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"100671","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-03-19"}],"displaytype":"detail","filename":"Cell_mf.pdf","filesize":[{"value":"3.4 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"Cell_mf","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/30336/files/Cell_mf.pdf"},"version_id":"6f53bca4-7901-4711-9d85-4e8ce23bce83"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"RNA polymerase II","subitem_subject_scheme":"Other"},{"subitem_subject":"RNAPII","subitem_subject_scheme":"Other"},{"subitem_subject":"ubiquitination of RNAPII","subitem_subject_scheme":"Other"},{"subitem_subject":"transcription coupled repair","subitem_subject_scheme":"Other"},{"subitem_subject":"TCR","subitem_subject_scheme":"Other"},{"subitem_subject":"nucleotide excision repair","subitem_subject_scheme":"Other"},{"subitem_subject":"NER","subitem_subject_scheme":"Other"},{"subitem_subject":"Cockayne syndrome","subitem_subject_scheme":"Other"},{"subitem_subject":"CS","subitem_subject_scheme":"Other"},{"subitem_subject":"UV-sensitive syndrome","subitem_subject_scheme":"Other"},{"subitem_subject":"UVsS","subitem_subject_scheme":"Other"},{"subitem_subject":"ChIP-seq","subitem_subject_scheme":"Other"},{"subitem_subject":"UVSSA","subitem_subject_scheme":"Other"},{"subitem_subject":"CRL/CSA/CSB","subitem_subject_scheme":"Other"},{"subitem_subject":"TFIIH","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["331"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2020-07-20"},"publish_date":"2020-07-20","publish_status":"0","recid":"30336","relation_version_is_last":true,"title":["Ubiquitination of DNA Damage-Stalled RNAPII Promotes Transcription-Coupled Repair"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T04:23:38.159770+00:00"}