{"_buckets": {"deposit": "0e4f50e7-a3a5-4e2c-83ec-01b5b30d8da1"}, "_deposit": {"id": "30546", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "30546"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00030546"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-08-09", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageStart": "e23388", "bibliographicVolumeNumber": "58", "bibliographic_titles": [{"bibliographic_title": "genesis"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "PR domain zinc finger protein 14 (PRDM14) plays an essential role in the development of primordial germ cells (PGCs) in mice. However, its functions in avian species remain unclear. In the present study, we used CRISPR/Cas9 to edit the PRDM14 locus in chickens in order to demonstrate its importance in development. The eGFP gene was introduced into the PRDM14 locus of cultured chicken PGCs to knockout PRDM14 and label PGCs. Chimeric chickens were established by a direct injection of eGFP knocked\u2010in (gene\u2010trapped) PGCs into the blood vessels of Hamburger\u2013Hamilton stages (HH\u2010stages) 13\u201316 chicken embryos. Gene\u2010trapped chickens were established by crossing a chimeric chicken with a wild\u2010type hen with very high efficiency. Heterozygous gene\u2010trapped chickens grew normally and SSEA\u20101\u2010positive cells expressed eGFP during HH\u2010stages 13\u201330. These results indicated the specific expression of eGFP within circulating PGCs and gonadal PGCs. At the blastodermal stage, the ratio of homozygous gene\u2010trapped embryos obtained by crossing heterozygous gene\u2010trapped roosters and hens was almost normal; however, all embryos died soon afterward, suggesting the important roles of PRDM14 in chicken early development.", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2021-08-09", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Wiley"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1002/dvg.23388", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\"This is the peer reviewed version of the following article: [Hagihara, Y, Okuzaki, Y, Matsubayashi, K, et al. Primordial germ cell\u2010specific expression of eGFP in transgenic chickens. genesis. 2020; 58:e23388. https://doi.org/10.1002/dvg.23388], which has been published in final form at [https://doi.org/10.1002/dvg.23388]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.\""}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "1526-954X", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hagihara, Yota"}], "nameIdentifiers": [{"nameIdentifier": "101697", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okuzaki, Yuya"}], "nameIdentifiers": [{"nameIdentifier": "101698", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Matsubayashi, Kazuma"}], "nameIdentifiers": [{"nameIdentifier": "101699", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Kaneoka, Hidenori"}], "nameIdentifiers": [{"nameIdentifier": "101700", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Takayuki"}], "nameIdentifiers": [{"nameIdentifier": "101701", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Iijima, Shinji"}], "nameIdentifiers": [{"nameIdentifier": "101702", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishijima, Ken\u2010ichi"}], "nameIdentifiers": [{"nameIdentifier": "101703", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-08-09"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "Hagihara_et_al_accepted_ver.pdf", "filesize": [{"value": "9.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 9900000.0, "url": {"label": "Hagihara_et_al_accepted_ver", "url": "https://nagoya.repo.nii.ac.jp/record/30546/files/Hagihara_et_al_accepted_ver.pdf"}, "version_id": "8d054991-87ed-49bd-a2b8-4a486302343b"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "Chicken", "subitem_subject_scheme": "Other"}, {"subitem_subject": "CRISPR/Cas9", "subitem_subject_scheme": "Other"}, {"subitem_subject": "eGFP", "subitem_subject_scheme": "Other"}, {"subitem_subject": "PRD\u039c14", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Primordial germ cells", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Primordial germ cell-specific expression of eGFP in transgenic chickens", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Primordial germ cell-specific expression of eGFP in transgenic chickens"}]}, "item_type_id": "10", "owner": "1", "path": ["643/666/667"], "permalink_uri": "http://hdl.handle.net/2237/00032731", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2020-10-15"}, "publish_date": "2020-10-15", "publish_status": "0", "recid": "30546", "relation": {}, "relation_version_is_last": true, "title": ["Primordial germ cell-specific expression of eGFP in transgenic chickens"], "weko_shared_id": null}