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Primordial germ cell-specific expression of eGFP in transgenic chickens
http://hdl.handle.net/2237/00032731
http://hdl.handle.net/2237/00032731fea2045b-42c9-4255-bbd1-b47eb2ee1e55
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2020-10-15 | |||||
タイトル | ||||||
タイトル | Primordial germ cell-specific expression of eGFP in transgenic chickens | |||||
言語 | en | |||||
著者 |
Hagihara, Yota
× Hagihara, Yota× Okuzaki, Yuya× Matsubayashi, Kazuma× Kaneoka, Hidenori× Suzuki, Takayuki× Iijima, Shinji× Nishijima, Ken‐ichi |
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アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is the peer reviewed version of the following article: [Hagihara, Y, Okuzaki, Y, Matsubayashi, K, et al. Primordial germ cell‐specific expression of eGFP in transgenic chickens. genesis. 2020; 58:e23388. https://doi.org/10.1002/dvg.23388], which has been published in final form at [https://doi.org/10.1002/dvg.23388]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chicken | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | CRISPR/Cas9 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | eGFP | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | PRDΜ14 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Primordial germ cells | |||||
抄録 | ||||||
内容記述 | PR domain zinc finger protein 14 (PRDM14) plays an essential role in the development of primordial germ cells (PGCs) in mice. However, its functions in avian species remain unclear. In the present study, we used CRISPR/Cas9 to edit the PRDM14 locus in chickens in order to demonstrate its importance in development. The eGFP gene was introduced into the PRDM14 locus of cultured chicken PGCs to knockout PRDM14 and label PGCs. Chimeric chickens were established by a direct injection of eGFP knocked‐in (gene‐trapped) PGCs into the blood vessels of Hamburger–Hamilton stages (HH‐stages) 13–16 chicken embryos. Gene‐trapped chickens were established by crossing a chimeric chicken with a wild‐type hen with very high efficiency. Heterozygous gene‐trapped chickens grew normally and SSEA‐1‐positive cells expressed eGFP during HH‐stages 13–30. These results indicated the specific expression of eGFP within circulating PGCs and gonadal PGCs. At the blastodermal stage, the ratio of homozygous gene‐trapped embryos obtained by crossing heterozygous gene‐trapped roosters and hens was almost normal; however, all embryos died soon afterward, suggesting the important roles of PRDM14 in chicken early development. | |||||
言語 | en | |||||
内容記述タイプ | Abstract | |||||
内容記述 | ||||||
内容記述 | ファイル公開:2021-08-09 | |||||
言語 | ja | |||||
内容記述タイプ | Other | |||||
出版者 | ||||||
言語 | en | |||||
出版者 | Wiley | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプresource | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
出版タイプ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | https://doi.org/10.1002/dvg.23388 | |||||
ISSN(print) | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 1526-954X | |||||
書誌情報 |
en : genesis 巻 58, 号 8, p. e23388, 発行日 2020-08-09 |
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著者版フラグ | ||||||
値 | author |