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  1. B300 農学部/生命農学研究科
  2. B300a 雑誌掲載論文
  3. 学術雑誌

Primordial germ cell-specific expression of eGFP in transgenic chickens

http://hdl.handle.net/2237/00032731
http://hdl.handle.net/2237/00032731
fea2045b-42c9-4255-bbd1-b47eb2ee1e55
名前 / ファイル ライセンス アクション
Hagihara_et_al_accepted_ver.pdf Hagihara_et_al_accepted_ver (9.9 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2020-10-15
タイトル
タイトル Primordial germ cell-specific expression of eGFP in transgenic chickens
言語 en
著者 Hagihara, Yota

× Hagihara, Yota

WEKO 101697

en Hagihara, Yota

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Okuzaki, Yuya

× Okuzaki, Yuya

WEKO 101698

en Okuzaki, Yuya

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Matsubayashi, Kazuma

× Matsubayashi, Kazuma

WEKO 101699

en Matsubayashi, Kazuma

Search repository
Kaneoka, Hidenori

× Kaneoka, Hidenori

WEKO 101700

en Kaneoka, Hidenori

Search repository
Suzuki, Takayuki

× Suzuki, Takayuki

WEKO 101701

en Suzuki, Takayuki

Search repository
Iijima, Shinji

× Iijima, Shinji

WEKO 101702

en Iijima, Shinji

Search repository
Nishijima, Ken‐ichi

× Nishijima, Ken‐ichi

WEKO 101703

en Nishijima, Ken‐ichi

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
権利
言語 en
権利情報 This is the peer reviewed version of the following article: [Hagihara, Y, Okuzaki, Y, Matsubayashi, K, et al. Primordial germ cell‐specific expression of eGFP in transgenic chickens. genesis. 2020; 58:e23388. https://doi.org/10.1002/dvg.23388], which has been published in final form at [https://doi.org/10.1002/dvg.23388]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.
キーワード
主題Scheme Other
主題 Chicken
キーワード
主題Scheme Other
主題 CRISPR/Cas9
キーワード
主題Scheme Other
主題 eGFP
キーワード
主題Scheme Other
主題 PRDΜ14
キーワード
主題Scheme Other
主題 Primordial germ cells
抄録
内容記述 PR domain zinc finger protein 14 (PRDM14) plays an essential role in the development of primordial germ cells (PGCs) in mice. However, its functions in avian species remain unclear. In the present study, we used CRISPR/Cas9 to edit the PRDM14 locus in chickens in order to demonstrate its importance in development. The eGFP gene was introduced into the PRDM14 locus of cultured chicken PGCs to knockout PRDM14 and label PGCs. Chimeric chickens were established by a direct injection of eGFP knocked‐in (gene‐trapped) PGCs into the blood vessels of Hamburger–Hamilton stages (HH‐stages) 13–16 chicken embryos. Gene‐trapped chickens were established by crossing a chimeric chicken with a wild‐type hen with very high efficiency. Heterozygous gene‐trapped chickens grew normally and SSEA‐1‐positive cells expressed eGFP during HH‐stages 13–30. These results indicated the specific expression of eGFP within circulating PGCs and gonadal PGCs. At the blastodermal stage, the ratio of homozygous gene‐trapped embryos obtained by crossing heterozygous gene‐trapped roosters and hens was almost normal; however, all embryos died soon afterward, suggesting the important roles of PRDM14 in chicken early development.
言語 en
内容記述タイプ Abstract
内容記述
内容記述 ファイル公開:2021-08-09
言語 ja
内容記述タイプ Other
出版者
言語 en
出版者 Wiley
言語
言語 eng
資源タイプ
資源タイプresource http://purl.org/coar/resource_type/c_6501
タイプ journal article
出版タイプ
出版タイプ AM
出版タイプResource http://purl.org/coar/version/c_ab4af688f83e57aa
DOI
関連タイプ isVersionOf
識別子タイプ DOI
関連識別子 https://doi.org/10.1002/dvg.23388
ISSN(print)
収録物識別子タイプ PISSN
収録物識別子 1526-954X
書誌情報 en : genesis

巻 58, 号 8, p. e23388, 発行日 2020-08-09
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