{"_buckets": {"deposit": "74ef999e-c9ba-4795-9aee-1e5dbca4b2f4"}, "_deposit": {"id": "31435", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "31435"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00031435"}, "item_10_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2020-10-16", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "2713", "bibliographicPageStart": "2703", "bibliographicVolumeNumber": "9", "bibliographic_titles": [{"bibliographic_title": "ACS Synthetic Biology"}]}]}, "item_10_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "Preventing the escape of hazardous genes from genetically modified organisms (GMOs) into the environment is one of the most important issues in biotechnology research. Various strategies were developed to create \u201cgenetic firewalls\u201d that prevent the leakage of GMOs; however, they were not specially designed to prevent the escape of genes. To address this issue, we developed amino acid (AA)-swapped genetic codes orthogonal to the standard genetic code, namely SL (Ser and Leu were swapped) and SLA genetic codes (Ser, Leu, and Ala were swapped). From mRNAs encoded by the AA-swapped genetic codes, functional proteins were only synthesized in translation systems featuring the corresponding genetic codes. These results clearly demonstrated the orthogonality of the AA-swapped genetic codes against the standard genetic code and their potential to function as \u201cgenetic firewalls for genes\u201d. Furthermore, we propose \u201ca codon-bypass strategy\u201d to develop a GMO with an AA-swapped genetic code.", "subitem_description_type": "Abstract"}]}, "item_10_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u30d5\u30a1\u30a4\u30eb\u516c\u958b\uff1a2021-10-16", "subitem_description_type": "Other"}]}, "item_10_publisher_32": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "ACS Publications"}]}, "item_10_relation_11": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "https://doi.org/10.1021/acssynbio.0c00196", "subitem_relation_type_select": "DOI"}}]}, "item_10_rights_12": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u201cThis document is the Accepted Manuscript version of a Published Work that appeared in final form in [ACS Synthetic Biology], copyright \u00a9 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-NB9DN3ZGG4GRS9ETGPWT].\u201d"}]}, "item_10_select_15": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "author"}]}, "item_10_source_id_61": {"attribute_name": "ISSN\uff08print\uff09", "attribute_value_mlt": [{"subitem_source_identifier": "2161-5063", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Fujino, Tomoshige"}], "nameIdentifiers": [{"nameIdentifier": "103690", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Tozaki, Masahiro"}], "nameIdentifiers": [{"nameIdentifier": "103691", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Murakami, Hiroshi"}], "nameIdentifiers": [{"nameIdentifier": "103692", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2021-10-16"}], "displaytype": "detail", "download_preview_message": "Download / Preview is available from 2021/10/16.", "file_order": 0, "filename": "AS_genetic_code_article_210208.pdf", "filesize": [{"value": "5.7 MB"}], "format": "application/pdf", "future_date_message": "Download is available from 2021/10/16.", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 5700000.0, "url": {"label": "AS_genetic_code_article_210208", "url": "https://nagoya.repo.nii.ac.jp/record/31435/files/AS_genetic_code_article_210208.pdf"}, "version_id": "a5cdfd36-c6ed-4c5a-b772-aa108cc49e09"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "genetic code", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cell-free translation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "tRNA", "subitem_subject_scheme": "Other"}, {"subitem_subject": "aminoacyl-tRNA synthetase", "subitem_subject_scheme": "Other"}, {"subitem_subject": "genetic firewall", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "An Amino Acid-Swapped Genetic Code", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "An Amino Acid-Swapped Genetic Code"}]}, "item_type_id": "10", "owner": "1", "path": ["320/321/322"], "permalink_uri": "http://hdl.handle.net/2237/00033615", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_name_i18n": "\u516c\u958b\u65e5", "attribute_value": "2021-02-15"}, "publish_date": "2021-02-15", "publish_status": "0", "recid": "31435", "relation": {}, "relation_version_is_last": true, "title": ["An Amino Acid-Swapped Genetic Code"], "weko_shared_id": null}