{"created":"2021-03-01T06:39:51.761239+00:00","id":31435,"links":{},"metadata":{"_buckets":{"deposit":"9ab18c7a-1e3e-45b8-bb45-7d5c450a87ac"},"_deposit":{"id":"31435","owners":[],"pid":{"revision_id":0,"type":"depid","value":"31435"},"status":"published"},"_oai":{"id":"oai:nagoya.repo.nii.ac.jp:00031435","sets":["320:321:322"]},"author_link":["103690","103691","103692"],"item_10_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2020-10-16","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"10","bibliographicPageEnd":"2713","bibliographicPageStart":"2703","bibliographicVolumeNumber":"9","bibliographic_titles":[{"bibliographic_title":"ACS Synthetic Biology","bibliographic_titleLang":"en"}]}]},"item_10_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Preventing the escape of hazardous genes from genetically modified organisms (GMOs) into the environment is one of the most important issues in biotechnology research. Various strategies were developed to create “genetic firewalls” that prevent the leakage of GMOs; however, they were not specially designed to prevent the escape of genes. To address this issue, we developed amino acid (AA)-swapped genetic codes orthogonal to the standard genetic code, namely SL (Ser and Leu were swapped) and SLA genetic codes (Ser, Leu, and Ala were swapped). From mRNAs encoded by the AA-swapped genetic codes, functional proteins were only synthesized in translation systems featuring the corresponding genetic codes. These results clearly demonstrated the orthogonality of the AA-swapped genetic codes against the standard genetic code and their potential to function as “genetic firewalls for genes”. Furthermore, we propose “a codon-bypass strategy” to develop a GMO with an AA-swapped genetic code.","subitem_description_language":"en","subitem_description_type":"Abstract"}]},"item_10_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"ファイル公開：2021-10-16","subitem_description_language":"ja","subitem_description_type":"Other"}]},"item_10_publisher_32":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"ACS Publications","subitem_publisher_language":"en"}]},"item_10_relation_11":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1021/acssynbio.0c00196","subitem_relation_type_select":"DOI"}}]},"item_10_rights_12":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"“This document is the Accepted Manuscript version of a Published Work that appeared in final form in [ACS Synthetic Biology], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [https://pubs.acs.org/articlesonrequest/AOR-NB9DN3ZGG4GRS9ETGPWT].”","subitem_rights_language":"en"}]},"item_10_select_15":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"author"}]},"item_10_source_id_61":{"attribute_name":"ISSN（print）","attribute_value_mlt":[{"subitem_source_identifier":"2161-5063","subitem_source_identifier_type":"PISSN"}]},"item_1615787544753":{"attribute_name":"出版タイプ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_access_right":{"attribute_name":"アクセス権","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Fujino, Tomoshige","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"103690","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Tozaki, Masahiro","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"103691","nameIdentifierScheme":"WEKO"}]},{"creatorNames":[{"creatorName":"Murakami, Hiroshi","creatorNameLang":"en"}],"nameIdentifiers":[{"nameIdentifier":"103692","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2021-10-16"}],"displaytype":"detail","filename":"AS_genetic_code_article_210208.pdf","filesize":[{"value":"5.7 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"AS_genetic_code_article_210208","objectType":"fulltext","url":"https://nagoya.repo.nii.ac.jp/record/31435/files/AS_genetic_code_article_210208.pdf"},"version_id":"436ee3df-7d92-4d07-9750-15588c3f2730"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"genetic code","subitem_subject_scheme":"Other"},{"subitem_subject":"cell-free translation","subitem_subject_scheme":"Other"},{"subitem_subject":"tRNA","subitem_subject_scheme":"Other"},{"subitem_subject":"aminoacyl-tRNA synthetase","subitem_subject_scheme":"Other"},{"subitem_subject":"genetic firewall","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"An Amino Acid-Swapped Genetic Code","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"An Amino Acid-Swapped Genetic Code","subitem_title_language":"en"}]},"item_type_id":"10","owner":"1","path":["322"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2021-02-15"},"publish_date":"2021-02-15","publish_status":"0","recid":"31435","relation_version_is_last":true,"title":["An Amino Acid-Swapped Genetic Code"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2023-01-16T05:13:05.415486+00:00"}