@article{oai:nagoya.repo.nii.ac.jp:00003920, author = {Kojima, Takashi and Nagata, Hiroshi and Tokuyama, Yutaka and Sano, Masaaki and Sasanabe, Ryuichiro and Suzumura, kazuyoshi and Kanemitsu, Taiseki and Naruse, Takayoshi}, issue = {1-2}, journal = {Nagoya Journal of Medical Science}, month = {May}, note = {We observed that mouse spleen cells form rosettes with autologous red blood cells (RBCs) and that rosette-formation was suppressed by anti-Aspergillus niger glucose oxidase monoclonal antibody (mAb).1) In the present study, we investigated whether RBCs of species besides mice have the structure recognized by anti-A. niger glucose oxidase mAb by using rosette-formation and complement-mediated hemolysis. Lysates of monkey and human RBCs did not suppress rosette-formation whereas autologous (mouse), rat and sheep RBC lysates partially suppressed rosette-formation. Those lysates exerted their suppressive activity after they had been treated at 56°C for 30 min. A. niger glucose oxidase also suppressed rosette-formation with or without treatment at 56°C for 30 min. Alternatively, anti-A. niger glucose oxidase mAb lysed mouse, rat and sheep RBCs but not human RBCs with complement. These findings suggest that the cell surfaces of mouse, rat and sheep RBCs have a structure which can be recognized by anti-A. niger glucose oxidase mAb while the cell surfaces of monkey and human RBCs do not.}, pages = {57--61}, title = {A discussion of anti-aspergillus niger glucose oxidase monoclonal antibody reactivity to red blood cells of several species}, volume = {63}, year = {2000} }