@article{oai:nagoya.repo.nii.ac.jp:00003921,
 author = {Sugihara, Takuro and Takahashi, Isao and Kojima, Tetsuhito and Okamoto, Yoshihiro and Yamamoto, Koji and Kamiya, Tadashi and Matsushita, Tadashi and Saito, Hidehiko},
 issue = {1-2},
 journal = {Nagoya Journal of Medical Science},
 month = {May},
 note = {Eleven Japanese hemophilia A patients with anti-factor VIII (FVIII)a) inhibitors were studied to localize both their inhibitory antibody epitopes and their genotypes. The inhibitor epitopes were studied in nine severe hemophilia A patients by means of a scanning method using the oligopeptide panel covering the FVIII polypeptides without the B domain. The 107 15 mer-peptides were synthesized on solid-phase pins and analyzed for their reactivity with diluted patient plasma. As indicated previously, a series of peptides corresponding to the A2 and C2 domains were recognized by plasma antibodies from 2 patients and 4 patients, respectively. In contrast, all the antibodies bound to several epitopes in the A3 domain, while an epitope 1809-1821 covering the putative factor IX binding site was found in 3 patients. Southern blotting analysis showed that 8 out of 11 patients had either gene deletions or inversions of the FVIII gene, indicating a higher proportion of gross gene alterations in inhibitor-positive hemophilia A patients. However, the correlation of gene abnormality type with epitope location was not fully established.},
 pages = {25--39},
 title = {Identification of plasma antibody epitopes and gene abnormalities in Japanese hemophilia a patients with factor VIII inhibitor},
 volume = {63},
 year = {2000}
}