{"_buckets": {"deposit": "7b46f436-6802-4f71-8ccf-b7fd3f8e542e"}, "_deposit": {"created_by": 17, "id": "3928", "owners": [17], "pid": {"revision_id": 0, "type": "depid", "value": "3928"}, "status": "published"}, "_oai": {"id": "oai:nagoya.repo.nii.ac.jp:00003928", "sets": []}, "author_link": ["10017", "10018", "10019"], "item_1615768549627": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_9_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2000-11", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "3-4", "bibliographicPageEnd": "113", "bibliographicPageStart": "99", "bibliographicVolumeNumber": "63", "bibliographic_titles": [{"bibliographic_title": "Nagoya Journal of Medical Science", "bibliographic_titleLang": "en"}]}]}, "item_9_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "The catalytic subunit of human DNA polymerase (pol) δ, p125, was expressed in recombinant baculovirus-infected insect cells, separated from a baculovirus-encoded DNA polymerase, and was purified to homogeneity by affinity trapping with a histidine-octapeptide at the C-terminus of p125 as the ligand. Purified p125 showed DNA polymerase activity resembling conventionally purified calf thymus pol δ. However, the two differed in four ways: 1) the specific activity of recombinant p125 was one quarter of the calf thymus pol δ; 2) the recombinant p125 was relatively resistant to aphidicolin; 3) the apparent Km for dTTP of the recombinant p125 was estimated at 33 μM, 15-fold the value for calf thymus pol δ; and 4) the recombinant p125 was not stimulated by recombinant PCNA, while activity of calf thymus pol δ increased 150-fold in response. Furthermore, PCNA did not stimulate either the p125 incubated with p50, a small subunit of pol δ, or co-expressed with p50 in insect cells. The full length recombinant p125 migrated slightly faster than pol δ from human cell lines, Jurkat or HeLa, upon SDS-polyacrylamide gel electrophoresis, suggesting a post-translational modification. The results indicate that in vivo assembly of the fully active complex of pol δ requires factors in addition to p125 and p50 subunits, and/or a post-translational modification of p125.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_9_identifier_60": {"attribute_name": "URI", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/2237/5358"}]}, "item_9_identifier_registration": {"attribute_name": "ID登録", "attribute_value_mlt": [{"subitem_identifier_reg_text": "10.18999/nagjms.63.3-4.99", "subitem_identifier_reg_type": "JaLC"}]}, "item_9_publisher_32": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Nagoya University School of Medicine", "subitem_publisher_language": "en"}]}, "item_9_select_15": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_9_source_id_61": {"attribute_name": "ISSN（Online）", "attribute_value_mlt": [{"subitem_source_identifier": "2186-3326", "subitem_source_identifier_type": "EISSN"}]}, "item_9_source_id_7": {"attribute_name": "ISSN（print）", "attribute_value_mlt": [{"subitem_source_identifier": "0027-7622", "subitem_source_identifier_type": "PISSN"}]}, "item_9_text_14": {"attribute_name": "フォーマット", "attribute_value_mlt": [{"subitem_text_value": "application/pdf"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Suzuki, Susumu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "10017", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Suzuki, Motoshi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "10018", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yoshida, Shonen", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "10019", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2018-02-19"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "nj6333.pdf", "filesize": [{"value": "154.0 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 154000.0, "url": {"label": "nj6333.pdf", "objectType": "fulltext", "url": "https://nagoya.repo.nii.ac.jp/record/3928/files/nj6333.pdf"}, "version_id": "b5191919-8b78-4238-a0a9-335f74f0a641"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "DNA polymerase δ catalytic subunit", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DNA polymerase δ small subunit", "subitem_subject_scheme": "Other"}, {"subitem_subject": "proliferating cell nuclear antigen", "subitem_subject_scheme": "Other"}, {"subitem_subject": "recombinant baculovirus", "subitem_subject_scheme": "Other"}, {"subitem_subject": "aphidicolin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Characterization of the human DNA polymerase of catalyticsubunit expressed by a recombinant baculovirus", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Characterization of the human DNA polymerase of catalyticsubunit expressed by a recombinant baculovirus", "subitem_title_language": "en"}]}, "item_type_id": "9", "owner": "17", "path": ["512"], "permalink_uri": "https://doi.org/10.18999/nagjms.63.3-4.99", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2006-02-03"}, "publish_date": "2006-02-03", "publish_status": "0", "recid": "3928", "relation": {}, "relation_version_is_last": true, "title": ["Characterization of the human DNA polymerase of catalyticsubunit expressed by a recombinant baculovirus"], "weko_shared_id": -1}